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. 2016 Feb 17;5:e11999. doi: 10.7554/eLife.11999
Reagent Type Manufacturer Catalog # Comments
D04 cells Cells Provided by Chris Schmidt, Queensland Institute of
Medical Research (QIMR) Berghofer, Australia
SB590885 Drug Selleckchem S2220 *Replaces Plexxion 885-A
DMSO Reagent Fisher Scientific D128-500 Original not specified
RPMI Media Life Technologies 21875-034
FBS Reagent Life Technologies 10270106
Effectene Transfection Reagent Reagent Qiagen 301425 Replaces Cell Line Nucleofector
Kit V (10 RCT) Lonza VACA1003
35 mm culture dishes Materials Corning CLS430165 Original not specified
Myc-CRAFWT vector Plasmid Shared by original authors
Myc-CRAFR89L vector Plasmid Shared by original authors
Empty vector Plasmid Shared by original authors
PBS Reagent Gibco 10010-023 Original not specified
Tris-HCl Chemical Specific brand information will be left up to the discretion
of the replicating lab and recorded later
NaCl Chemical
Igepal Chemical
Na3VO4 Chemical
NaF Chemical
Leupeptin Chemical
Rabbit α myc Antibody Abcam ab9106
Mouse α BRAF (F-7) Antibody Santa Cruz Biotechnology sc-5284
Mouse α myc (9B11) (HRP conjugate) Antibody Cell Signaling Technology 2040
Protein G sepharose beads Materials Sigma P3296
NuPAGE Sample buffer Buffer Invitrogen NP0007 Original not specified
SDS-Page gel (4–12%) Western blot reagent Invitrogen NP0322BOX Original not specified
Nitrocellulose membrane (iBlot) Western blot reagent Invitrogen IB301002 Original not specified
Ponceau stain Western blot reagent Sigma-Aldrich P7170-1L Original not specified
Tris Chemical Sigma-Aldrich T6066 Original not specified
Tween-20 Chemical Sigma-Aldrich P1379 Original not specified
HPR-conjugated secondary antibody Western blot reagent Bio-Rad 170-5047 Original not specified
ECL Detection Kit Western blot reagent Invitrogen 32132 Original not specified