Reagent	Type	Manufacturer	Catalog #	Comments
D04 cells	Cells	Provided by Chris Schmidt, Queensland Institute of Medical Research (QIMR) Berghofer, Australia
SB590885	Drug	Selleckchem	S2220	*Replaces Plexxion 885-A
DMSO	Reagent	Fisher Scientific	D128-500	Original not specified
RPMI	Media	Life Technologies	21875-034
FBS	Reagent	Life Technologies	10270106
Effectene Transfection Reagent	Reagent	Qiagen	301425	Replaces Cell Line Nucleofector Kit V (10 RCT) Lonza VACA1003
35-mm culture dishes	Materials	Corning	CLS430165	Original not specified
Myc-BRAFWT vector	Plasmid	Shared by original authors
Myc-BRAFR188L vector	Plasmid	Shared by original authors
Empty vector	Plasmid	Shared by original authors
PBS	Reagent	Gibco	10010-023	Original not specified
Tris-HCl	Chemical	Specific brand information will be left up to the discretion of the replicating lab and recorded later
NaCl	Chemical
Igepal	Chemical
Na3VO4	Chemical
NaF	Chemical
Leupeptin	Chemical
Rabbit anti-myc	Antibody	Abcam	ab9106
mouse anti-CRAF	Antibody	BD Transduction Laboratories	610152
Mouse α myc (9B11) (HRP conjugate)	Antibody	Cell Signaling Technology	2040
Protein G sepharose beads	Materials	Sigma	P3296
NuPAGE Sample buffer	Buffer	Invitrogen	NP0007	Original not specified
SDS-Page gel (4–12%)	Western blot reagent	Invitrogen	NP0322BOX	Original not specified
Nitrocellulose membrane (iBlot)	Western blot reagent	Invitrogen	IB301002	Original not specified
Ponceau stain	Western blot reagent	Sigma-Aldrich	P7170-1L	Original not specified
Tris	Chemical	Sigma-Aldrich	T6066	Original not specified
Tween-20	Chemical	Sigma-Aldrich	P1379	Original not specified
HPR-conjugated secondary antibody	Western blot reagent	Bio-Rad	170-5047	Original not specified
ECL Detection Kit	Western blot reagent	Invitrogen	32132	Original not specified