Reagent	Type	Manufacturer	Catalog #	Comments
D04 cells	Cells	Provided by Chris Schmidt, Queensland Institute of Medical Research (QIMR) Berghofer, Australia
RPMI	Media	Life Technologies	21875-034
FBS	Reagent	Life Technologies	10270106
Effectene Transfection Reagent	Reagent	Qiagen	301425	Replaces Cell Line Nucleofector Kit V (10 RCT) Lonza VACA1003
Myc-BRAFWT vector	Plasmid	Shared by original author
Myc-BRAFD594A vector	Plasmid	Shared by original author
Empty vector	Plasmid	Shared by original author
35 mm culture dishes	Materials
PBS	Reagent	Gibco	10010-023	Original not specified
Tris-HCl	Chemical	Specific brand information will be left up to the discretion of the replicating lab and recorded later
NaCl	Chemical
Igepal	Chemical
Na3VO4	Chemical
NaF	Chemical
Leupeptin	Chemical
Rabbit α myc	Antibody	Abcam	ab9106
Mouse α CRAF (for Western blotting)	Antibody	BD Transduction Laboratories	610152
Mouse α myc (9B11) (HRP conjugate)	Antibody	Cell Signaling Technology	2040
Protein G sepharose beads	Materials	Sigma	P3296
NuPAGE Sample buffer	Buffer	Invitrogen	NP0007	Original not specified
SDS-Page gel (4–12%)	Western blot reagent	Invitrogen	NP0322BOX	Original not specified
Nitrocellulose membrane (iBlot)	Western blot reagent	Invitrogen	IB301002	Original not specified
Ponceau stain	Western blot reagent	Sigma-Aldrich	P7170-1L	Original not specified
Tris	Chemical	Sigma-Aldrich	T6066	Original not specified
Tween-20	Chemical	Sigma-Aldrich	P1379	Original not specified
HPR-conjugated secondary antibody	Western blot reagent	Bio-Rad	170-5047	Original not specified
ECL Detection Kit	Western blot reagent	Invitrogen	32132	Original not specified