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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1971 Apr;24(3):270–282. doi: 10.1136/jcp.24.3.270

Structure and ultrastructure of the labial salivary glands in patients with cystic fibrosis

R G Doggett 1,2,3,1, B Bentinck 1,2,3,2, G M Harrison 1,2,3
PMCID: PMC476967  PMID: 4324770

Abstract

The morphology of the labial salivary glands in subjects with and without cystic fibrosis has been studied by light and electron microscopy. The terms `light' cell, `dark' cell, `clear' cell, and `myoepithelial' cell are used to describe the four cell types distinguished at the level of the electron microscope. The dark cells were found to stain specifically with alcian blue at and above pH 1·0, and the light cells to stain specifically using the periodic acid-Schiff (PAS) technique. This staining reaction suggests that an acidic mucosubstance is being produced by the dark cells and a neutral mucosubstance by the light cells. While no marked morphological differences were observed in the labial glands of patients with cystic fibrosis when contrasted with normal controls, it was observed that the cells from patients with cystic fibrosis contained quantitatively more mucus.

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Selected References

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  1. "ADULT" mucoviscidosis. Am J Med. 1959 Sep;27:483–493. [PubMed] [Google Scholar]
  2. CHERNICK W. S., BARBERO G. J., PARKINS F. M. Studies on submaxillary saliva in cystic fibrosis. J Pediatr. 1961 Dec;59:890–898. doi: 10.1016/s0022-3476(61)80319-3. [DOI] [PubMed] [Google Scholar]
  3. CHERNICK W. S., EICHEL H. J., BARBERO G. J. SUBMAXILLARY SALIVARY ENZYMES AS A MEASURE OF GLANDULAR ACTIVITY IN CYSTIC FIBROSIS. J Pediatr. 1964 Nov;65:694–700. doi: 10.1016/s0022-3476(64)80153-0. [DOI] [PubMed] [Google Scholar]
  4. Cezeaux G., Jr, Telford J., Harrison G., Keats A. S. Bronchial lavage in cystic fibrosis. A comparison of agents. JAMA. 1967 Jan 2;199(1):15–18. [PubMed] [Google Scholar]
  5. DARLING R. C., DISANT'AGNESE P. A., PERERA G. A., ANDERSEN D. H. Electrolyte abnormalities of the sweat in fibrocystic disease of the pancreas. Am J Med Sci. 1953 Jan;225(1):67–70. [PubMed] [Google Scholar]
  6. HURLEY H. J., Jr, SHELLEY W. B. The role of the myoepithelium of the human apocrine sweat gland. J Invest Dermatol. 1954 Feb;22(2):143–156. doi: 10.1038/jid.1954.19. [DOI] [PubMed] [Google Scholar]
  7. Hunton D. B., Long W. K., Tsumagari H. Y. Meconium ileus equivalent: an adult complication of fibrocystic disease. Gastroenterology. 1966 Jan;50(1):99–106. [PubMed] [Google Scholar]
  8. LEV R., SPICER S. S. A HISTOCHEMICAL COMPARISON OF HUMAN EPITHELIAL MUCINS IN NORMAL AND IN HYPERSECRETORY STATES INCLUDING PANCREATIC CYSTIC FIBROSIS. Am J Pathol. 1965 Jan;46:23–47. [PMC free article] [PubMed] [Google Scholar]
  9. MARKS B. L., ANDERSON C. M. Fibrocystic disease of the pancreas in a man aged 46. Lancet. 1960 Feb 13;1(7120):365–367. doi: 10.1016/s0140-6736(60)90289-0. [DOI] [PubMed] [Google Scholar]
  10. MAYOR H. D., HAMPTON J. C., ROSARIO B. A simple method for removing the resin from epoxy-embedded tissue. J Biophys Biochem Cytol. 1961 Apr;9:909–910. doi: 10.1083/jcb.9.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MESKIN L. H., BERNARD B., WARWICK W. J. BIOPSY OF THE LABIAL MUCOUS SALIVARY GLANDS IN CYSTIC FIBROSIS. JAMA. 1964 Apr 6;188:82–83. doi: 10.1001/jama.1964.03060270088026. [DOI] [PubMed] [Google Scholar]
  12. MONTAGNA W., CHASE H. B., LOBITZ W. C., Jr Histology and cytochemistry of human skin. IV. The eccrine sweat glands. J Invest Dermatol. 1953 Jun;20(6):415–423. doi: 10.1038/jid.1953.52. [DOI] [PubMed] [Google Scholar]
  13. MUNGER B. L., BRUSILOW S. W., COOKE R. E. An electron microscopic study of eccrine sweat glands in patients with cystic fibrosis of the pancreas. J Pediatr. 1961 Oct;59:497–511. doi: 10.1016/s0022-3476(61)80233-3. [DOI] [PubMed] [Google Scholar]
  14. Mandel I. D., Kutscher A., Denning C. R., Thompson R. H., Jr, Zegarelli E. V. Salivary studies in cystic fibrosis. Am J Dis Child. 1967 Apr;113(4):431–438. doi: 10.1001/archpedi.1967.02090190077005. [DOI] [PubMed] [Google Scholar]
  15. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PARKS H. F. Morphological study of the extrusion of secretory materials by the parotid glands of mouse and rat. J Ultrastruct Res. 1962 Jun;6:449–465. doi: 10.1016/s0022-5320(62)80002-1. [DOI] [PubMed] [Google Scholar]
  17. PARKS H. F. On the fine structure of the parotid gland of mouse and rat. Am J Anat. 1961 May;108:303–329. doi: 10.1002/aja.1001080306. [DOI] [PubMed] [Google Scholar]
  18. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCOTT B. L., PEASE D. C. Electron microscopy of the salivary and lacrimal glands of the rat. Am J Anat. 1959 Jan;104:115–161. doi: 10.1002/aja.1001040106. [DOI] [PubMed] [Google Scholar]
  21. Spicer S. S., Leppi T. J., Stoward P. J. Suggestions for a histochemical terminology of carbohydrate-rich tissue components. J Histochem Cytochem. 1965 Sep-Oct;13(7):599–603. doi: 10.1177/13.7.599. [DOI] [PubMed] [Google Scholar]
  22. Sweney L. R., Hedrick M. C., Meskin L. H., Warwick W. J. The involvement of the labial mucous salivary gland in patients with cystic fibrosis. II. The heterozygote state. Pediatrics. 1967 Sep;40(3):421–424. [PubMed] [Google Scholar]
  23. Tandler B., Denning C. R., Mandel I. D., Kutscher A. H. Ultrastructure of human labial salivary glands. I. Acinar secretory cells. J Morphol. 1969 Apr;127(4):383–407. doi: 10.1002/jmor.1051270402. [DOI] [PubMed] [Google Scholar]
  24. WARWICK W. J., BERNARD B., MESKIN L. H. THE INVOLVEMENT OF THE LABIAL MUCOUS SALIVARY GLAND IN PATIENTS WITH CYSTIC FIBROSIS. Pediatrics. 1964 Nov;34:621–628. [PubMed] [Google Scholar]

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