Figure 1. miR-214 is up-regulated in LAD and positively associated with metastasis.

A. The quantitation of miR-214 expression in human primary LAD cancer tissues (n = 22) and paracancerous tissues (n = 13). U6 was used as a loading control for miR-214. The relative expression levels were normalized to the mean value of all patients. B. The correlation between the miR-214 levels and clinical pathological parameters in LAD patients. C. miR-214 levels in primary lung tumors and matched lymph node metastases by real-time PCR (n = 15). D. miR-214 expression was detected among five LAD cell lines in a descending order of metastatic potential. E. 1 × 10⁶ SPC-A1, HCC827, A549 or NCI-H1650 cells were simultaneously injected into the right flank of SCID mice. Primary tumors were collected to investigate miR-214 expression levels by qRT-PCR (n = 6). All the experiments were performed at least three times and the data are expressed as the means ± SD. The statistical significance of differences was measured by unpaired student's t-test (A) and paired student's t-test (C).