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. 2015 Oct 7;6(36):38804–38815. doi: 10.18632/oncotarget.5661

Figure 2. FACS analysis of CD97 expression in AML cell lines with different FLT3 mutation state.

Figure 2

A. CD97 expression levels in FLT3-ITD carrying MV4-11 and MOLM-13 cells were significantly higher compared to EOL-1, OCI-AML3 and HL-60 cells. Treatment of MV4-11 and MOLM-13 cells with 0.5 μM of the tyrosine kinase inhibitor PKC412 or 0.1 μM SU5614 significantly decreased the CD97 expression whereas the low CD97 expression levels in EOL-1 cells were not affected by these inhibitors. CD97 levels in OCI-AML3 and HL-60 cells were even increased by PKC412 treatment. Data is represented as mean ± SEM of three independent experiments, p < 0.05 (*); p < 0.01 (**); p < 0.001 (***). B. One representative measurement for MV4-11 cells is displayed. Filled grey: isotype control; black: CD97-APC in DMSO treated control cells; red: CD97-APC in PKC412 treated cells; green: CD97-APC in SU5614 treated cells.