Figure 3. CD97 expression in MV4-11 cells can be knocke down by shRNA which also affects FLT3 expression.
A. Different shRNAs down-regulated CD97 expression with various efficiency as measured by flow cytometry using anti-CD97-APC antibodies in comparison to the isotype control IgG-APC (filled grey). One representative measurement is shown. B. The most effective shRNA targeting CD97 also down-regulated wildtype FLT3 (CD135) expression as shown by flow cytometry. One representative measurement is presented for CD97-APC (left) and CD135-APC (right).