Table 2. Comparison of microarray gene expression profiling and real-time RT-PCR for six selected genes.

Genes	Fold change
	Shikonin		Isobutyrylshikonin		β,β-Dimethylacrylshikonin		Isovalerylshikonin		2-Methylbutyrylshikonin
	Microarray	RT-PCR	Microarray	RT-PCR	Microarray	RT-PCR	Microarray	RT-PCR	Microarray	RT-PCR
JUN	5.8	30.67	7.1	160.45	5.1	19.37	7.7	37.24	4.7	32.05
GABARAPL1	5.4	78.16	5.2	78.14	4.1	56.77	4.5	96.57	4.3	112.43
LY96	9	69.84	12.4	161.44	9.8	49.32	4.7	93.54	11.2	103.92
IFITM2	−3.5	−1.05	−4.2	−1.31	−3.8	−1.59	−5.1	−1.27	−3.2	−1.32
MYC	−5.4	−2.37	−10.3	−2.30	−10.4	−5.97	−9.9	−5.26	−8.3	−2.20
MS4AS	−3.6	−1.72	−7.0	−2.62	−6.8	−10.03	−5.3	−4.63	−8.3	–1.24
R-value	0.9011		0.9386		0.8662		0.7954		0.8480

The correlation coefficient (R-value) between mRNA expression values determined by microarray hybridization and real-time RT-PCR were caculated with Pearson correlation test