Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1971 Sep;24(6):487–490. doi: 10.1136/jcp.24.6.487

Simplified organ cultures of human embryo trachea in the diagnosis of viral respiratory disease of children

A P C H Roome 1,2,3, Valerie Dickinson 1,2,3, E O Caul 1,2,3
PMCID: PMC477077  PMID: 4328702

Abstract

Organ cultures of human embryonic trachea in test tubes were used as an adjunct to tissue cultures in the isolation of respiratory viruses from children in hospital. Fifty-one viruses were obtained from 127 specimens, giving an isolation rate of 40%. Fifteen viruses were isolated from the original tissue cultures and also after passage through organ culture. Thirty viruses were isolated from the original tissue culture only, and six viruses only from organ culture (three para-influenza, one influenza A, and one rhinovirus). An increase of 5% in virus isolation rate over that of standard tissue culture was obtained.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler M. Isolation and growth of mycoplasma in human embryo trachea cultures. Nature. 1969 Nov 8;224(5219):605–606. doi: 10.1038/224605a0. [DOI] [PubMed] [Google Scholar]
  2. Cherry J. D., Taylor-Robinson D. Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies. Appl Microbiol. 1970 Apr;19(4):658–662. doi: 10.1128/am.19.4.658-662.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOORN B. RESPIRATORY VIRUSES IN MODEL EXPERIMENTS. Acta Otolaryngol Suppl. 1964;188:SUPPL 188–188:138+. doi: 10.3109/00016486409134552. [DOI] [PubMed] [Google Scholar]
  4. HOORN B., TYRRELL D. A. ON THE GROWTH OF CERTAIN "NEWER" RESPIRATORY VIRUSES IN ORGAN CULTURES. Br J Exp Pathol. 1965 Apr;46:109–118. [PMC free article] [PubMed] [Google Scholar]
  5. Harnett G. B., Hooper W. L. Test-tube organ cultures of ciliated epithelium for the isolation of respiratory viruses. Lancet. 1968 Feb 17;1(7538):339–340. doi: 10.1016/s0140-6736(68)90800-3. [DOI] [PubMed] [Google Scholar]
  6. Higgins P. G., Ellis E. M., Woolley D. A. A comparative study of standard methods and organ culture for the isolation of respiratory viruses. J Med Microbiol. 1969 May;2(2):109–115. doi: 10.1099/00222615-2-2-109. [DOI] [PubMed] [Google Scholar]
  7. Higgins P. G. The isolation of viruses from acute respiratory infections. V. The use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. Mon Bull Minist Health Public Health Lab Serv. 1966 Dec;25:283–288. [PubMed] [Google Scholar]
  8. Hoorn B., Tyrrell D. A. Organ cultures in virology. Prog Med Virol. 1969;11:408–450. [PubMed] [Google Scholar]
  9. McIntosh K., Dees J. H., Becker W. B., Kapikian A. Z., Chanock R. M. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc Natl Acad Sci U S A. 1967 Apr;57(4):933–940. doi: 10.1073/pnas.57.4.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. TYRRELL D. A., BYNOE M. L. CULTIVATION OF A NOVEL TYPE OF COMMON-COLD VIRUS IN ORGAN CULTURES. Br Med J. 1965 Jun 5;1(5448):1467–1470. doi: 10.1136/bmj.1.5448.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tyrrell D. A., Blamire C. J. Improvements in a method of growing respiratory viruses in organ cultures. Br J Exp Pathol. 1967 Apr;48(2):217–227. [PMC free article] [PubMed] [Google Scholar]
  12. Tyrrell D. A., Bynoe M. L. Cultivation of viruses from a high proportion of patients with colds. Lancet. 1966 Jan 8;1(7428):76–77. doi: 10.1016/s0140-6736(66)92364-6. [DOI] [PubMed] [Google Scholar]
  13. Tyrrell D. A., Bynoe M. L., Hoorn B. Cultivation of "difficult" viruses from patients with common colds. Br Med J. 1968 Mar 9;1(5592):606–610. doi: 10.1136/bmj.1.5592.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tyrrell D. A., Hoorn B. The growth of some myxoviruses in organ cultures. Br J Exp Pathol. 1965 Oct;46(5):514–518. [PMC free article] [PubMed] [Google Scholar]
  15. Tyrrell D. A. Hunting common cold viruses by some new methods. J Infect Dis. 1970 May;121(5):561–571. doi: 10.1093/infdis/121.5.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Votava M., Tyrrell D. A. The possible value of certain cells for the propagation of respiratory viruses. Arch Gesamte Virusforsch. 1970;29(2):253–256. doi: 10.1007/BF01249310. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES