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. 2015 Oct 21;6(36):39247–39261. doi: 10.18632/oncotarget.5746

Figure 2. ERp57 modulates chemotherapy-induced apoptosis in a cell-type specific manner.

Figure 2

For all chemotherapy experiments control cells were treated with vehicle only (DMSO). A. Apoptosis was triggered in inducible HCT116shERp57 and MDA-MB-231shERp57 by treatment with etoposide 24 h after induction of the ERp57 knockdown. 48 h after treatment whole cell extracts were tested for caspase-3 activity. B. Cells were treated with 50 μM etoposide (Eto) 36 h after induction of ERp57 knockdown. 48 h after treatment apoptosis was quantified by staining with annexin V and PI and subsequent FACS analysis. Representative data of two experiments are shown. C. Cells treated as in (B) were subjected to Western blotting. D. Cells were treated with 50 μM 5-fluorouracil (5-FU) 24 h after induction of the ERp57 knockdown. 48 h after treatment caspase-3 activity was quantified. E. Cells were incubated with 50 μM 5-fluorouracil 36 h after induction of ERp57 knockdown. 48 h after treatment cells were stained with annexin V and PI for FACS. Representative data of two experiments are shown. F. In parallel, cell lysates were analysed by Western blotting.