Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1971 Sep;24(6):501–505. doi: 10.1136/jcp.24.6.501

Use of peroxidase-conjugated antiglobulin as an alternative to immunofluorescence for the detection of antinuclear factor in serum

J Dorling 1, G D Johnson 1, Janet A Webb 1, Mavis E Smith 1
PMCID: PMC477080  PMID: 4106192

Abstract

A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxidase method, in which an ordinary light microscope is employed, provides a satisfactory alternative to immunofluorescence for routine tests for antinuclear factor.

Full text

PDF
501

Images in this article

503Image
on p.503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beutner E. H., Holborow E. J., Johnson G. D. Quantitative studies of immunofluorescent staining. I. Analyses of mixed immunofluorescence. Immunology. 1967 Mar;12(3):327–337. [PMC free article] [PubMed] [Google Scholar]
  2. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davey F. R., Busch G. J. Immunohistochemistry of glomerulonephritis using horseradish peroxidase and fluorescein-labeled antibody: a comparison of two technics. Am J Clin Pathol. 1970 Apr;53(4):531–536. doi: 10.1093/ajcp/53.4.531. [DOI] [PubMed] [Google Scholar]
  4. GRAHAM R. C., Jr, LUNDHOLM U., KARNOVSKY M. J. CYTOCHEMICAL DEMONSTRATION OF PEROXIDASE ACTIVITY WITH 3-AMINO-9-ETHYLCARBAZOLE. J Histochem Cytochem. 1965 Feb;13:150–152. doi: 10.1177/13.2.150. [DOI] [PubMed] [Google Scholar]
  5. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  6. HOLBOROW E. J., WEIR D. M., JOHNSON G. D. A serum factor in lupus erythematosus with affinity for tissue nuclei. Br Med J. 1957 Sep 28;2(5047):732–734. doi: 10.1136/bmj.2.5047.732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoedemaeker P. J., Ito S. Ultrastructural localization of gastric parietal cell antigen with peroxidase-coupled antibody. Lab Invest. 1970 Feb;22(2):184–188. [PubMed] [Google Scholar]
  8. Johnson G. D., Beutner E. H., Holborow E. J. Developments in immunofluorescence: the need for standardization. J Clin Pathol. 1967 Sep;20(5):720–723. doi: 10.1136/jcp.20.5.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  10. Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
  11. O'Neill P., Johnson G. D. Multispot immunofluorescence: a simple semi-automatic method of processing large numbers of tests. J Clin Pathol. 1970 Mar;23(2):185–187. doi: 10.1136/jcp.23.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STRAUS W. FACTORS AFFECTING THE CYTOCHEMICAL REACTION OF PEROXIDASE WITH BENZIDINE AND THE STABILITY OF THE BLUE REACTION PRODUCT. J Histochem Cytochem. 1964 Jun;12:462–469. doi: 10.1177/12.6.462. [DOI] [PubMed] [Google Scholar]
  13. Straus W. Localization of the antigen in popliteal lymph nodes of rabbits during the formation of antibodies to horseradish peroxidase. J Histochem Cytochem. 1970 Feb;18(2):131–142. doi: 10.1177/18.2.131. [DOI] [PubMed] [Google Scholar]
  14. Straus W. Location of antibody to horseradish peroxidase in popliteal lymph nodes of rabbits during the primary and early secondary response. J Histochem Cytochem. 1970 Feb;18(2):120–130. doi: 10.1177/18.2.120. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES