Abstract
Inhibition of buffy layer peripheral leucocyte migration by tuberculin purified protein derivative (PPD) from micro-capillaries mounted in small tissue culture chambers correlates in all cases with a positive Mantoux test. This quick and reproducible test of cellular hypersensitivity in man requires only 5-15 ml of blood and is applicable for study in children and in clinical situations where repeated monitoring of cellular immunity may be required.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bendixen G., Soborg M. A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. Dan Med Bull. 1969 Jan;16(1):1–6. [PubMed] [Google Scholar]
- Brostoff J., Roitt I. M. Cell-mediated (delayed) hypersensitivity in patients with summer hay-fever. Lancet. 1969 Dec 13;2(7633):1269–1272. doi: 10.1016/s0140-6736(69)90807-1. [DOI] [PubMed] [Google Scholar]
- Dumonde D. C. 'Lymphokines': molecular mediators of cellular immune responses in animals and man. Proc R Soc Med. 1970 Sep;63(9):899–902. [PMC free article] [PubMed] [Google Scholar]
- Dumonde D. C., Maini R. N. The clinical significance of mediators of cellular immunity. Clin Allergy. 1971 Mar;1(1):123–139. doi: 10.1111/j.1365-2222.1971.tb02453.x. [DOI] [PubMed] [Google Scholar]
- Kaltreider H. B., Soghor D., Taylor J. B., Decker J. L. Capillary tube migration for detection of human delayed hypersensitivity: difficulties encountered with "buffy coat" cells and tuberculin antigen. J Immunol. 1969 Aug;103(2):179–184. [PubMed] [Google Scholar]
- Lockshin M. D. Failure to demonstrate leukocyte migration inhibition in human tuberculin hypersensitivity. Proc Soc Exp Biol Med. 1969 Dec;132(3):928–930. doi: 10.3181/00379727-132-34339. [DOI] [PubMed] [Google Scholar]
- Maini R. N., Stewart S. M., Dumonde D. C. Peripheral leucocyte migration. Inhibited by diphtheroid organisms isolated from patients with rheumatoid arthritis. Ann Rheum Dis. 1970 Sep;29(5):541–545. doi: 10.1136/ard.29.5.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith M. G., Eddleston A. L., Dominguez J. A., Evans D. B., Bewick M., Williams R. Changes in leucocyte migration after renal transplantation. Br Med J. 1969 Nov 1;4(5678):275–278. doi: 10.1136/bmj.4.5678.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soborg M. Interaction of human peripheral lymphocytes and granulocytes in the migration-inhibition reaction. Acta Med Scand. 1969 Mar;185(3):221–226. [PubMed] [Google Scholar]
- Williams M. H., Brostoff J., Roitt I. M. Possible role of Mycoplasma fermentans in pathogenesis of rheumatoid arthritis. Lancet. 1970 Aug 8;2(7667):277–280. doi: 10.1016/s0140-6736(70)91328-0. [DOI] [PubMed] [Google Scholar]