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. 1971 Dec;24(9):856–866. doi: 10.1136/jcp.24.9.856

Plasma calcium fractions and the protein-binding of calcium in normal subjects and in patients with hypercalcaemia and hypocalcaemia

M R Wills 1, M R Lewin 1
PMCID: PMC477199  PMID: 5139991

Abstract

A study is reported of the estimation of plasma calcium fractions and the calcium-binding affinity of plasma proteins in a total sample of 59 people, which included 29 normal subjects and 30 patients with either hypercalcaemia or hypocalcaemia. It was demonstrated that when the sample was considered as a whole there was a significant correlation between the total plasma calcium concentration and the ultrafiltrable, ionized, and protein-bound calcium fractions and between the ultrafiltrable and ionized fractions. We have also demonstrated that in patients with either hypercalcaemia or hypocalcaemia, including acidotic uraemics, the calcium-binding affinity of the plasma proteins did not differ significantly from that in normal subjects. A significant correlation was also found between the total plasma calcium concentration and the ultrafiltrable, ionized and protein-bound calcium fractions when the normal subjects and the groups of patients with hypercalcaemia and hypocalcaemia due to chronic renal failure were considered as separate groups. The group of patients with hypercalcaemia included patients both with hyperparathyroidism and with hypercalcaemia due to other causes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dale N. E., Kellerman G. M. The binding of calcium by the plasma proteins in hyperparathyroidism. Clin Sci. 1967 Jun;32(3):433–442. [PubMed] [Google Scholar]
  2. ETTORI J., SCOGGAN S. M. A determination of ionized calcium in serum by two- wavelength spectrophotometry of a metal indicator. Clin Chim Acta. 1961 Nov;6:861–865. doi: 10.1016/0009-8981(61)90175-9. [DOI] [PubMed] [Google Scholar]
  3. FANCONI A., ROSE G. A. The ionized, complexed, and protein-bound fractions of calcium in plasma; an investigation of patients with various diseases which affect calcium metabolism, with an additional study of the role of calcium ions in the prevention of tetany. Q J Med. 1958 Oct;27(108):463–494. [PubMed] [Google Scholar]
  4. FOWLER D. I., FONE D. J., COOKE W. T. The calcium fractions of plasma in hypercalcaemic conditions and in adult coeliac disease. Lancet. 1961 Aug 5;2(7197):284–287. doi: 10.1016/s0140-6736(61)90578-5. [DOI] [PubMed] [Google Scholar]
  5. HELLSTROM J. Observations regarding the prognosis and diagnosis of hyperparathyroidism. Cleve Clin Q. 1953 Jan;20(1):253–256. doi: 10.3949/ccjm.20.1.253. [DOI] [PubMed] [Google Scholar]
  6. HODGKINSON A., EDWARDS N. A. TOTAL AND ULTRAFILTERABLE SERUM CALCIUM IN PRIMARY HYPERPARATHYROIDISM. Br J Urol. 1963 Dec;35:445–456. doi: 10.1111/j.1464-410x.1963.tb11764.x. [DOI] [PubMed] [Google Scholar]
  7. HOPKINS T., HOWARD J. E., EISENBERG H. Ultrafiltration studies on calcium and phosphorus in human serum. Bull Johns Hopkins Hosp. 1952 Jul;91(1):1–21. [PubMed] [Google Scholar]
  8. Hollinrake K., Thomas P. K., Wills M. R., Baillod R. A. Observations on plasma magnesium levels in patients with uremic neuropathy under treatment by periodic hemodialysis. Neurology. 1970 Sep;20(9):939–942. doi: 10.1212/wnl.20.9.939. [DOI] [PubMed] [Google Scholar]
  9. Jacob E., Lim P., Khoo O. T. Serum ionized calcium level in chronic renal failure. Med J Aust. 1969 Jun 7;1(23):1172–1174. doi: 10.5694/j.1326-5377.1969.tb62252.x. [DOI] [PubMed] [Google Scholar]
  10. Kotzaurek R. Uber Erfahrungen mit neueren Untersuchungsmethoden bei Mineralstoffwechselstudien. II. Ionisiertes Calcium im Blutserum. Arch Klin Med. 1967 May 23;213(2):96–105. [PubMed] [Google Scholar]
  11. LLOYD H. M., ROSE G. A. Ionised, protein-bound, and complexed calcium in the plasma in primary hyperparathyroidism. Lancet. 1958 Dec 13;2(7059):1258–1261. doi: 10.1016/s0140-6736(58)91390-4. [DOI] [PubMed] [Google Scholar]
  12. LLOYD H. M., ROSE G. A., SMEENK D. The ability of plasma proteins to bind calcium in normal subjects, in patients with primary hyperparathyroidism both pre- and post-operatively, and in other hypercalcaemic conditions. Clin Sci. 1962 Jun;22:353–362. [PubMed] [Google Scholar]
  13. LOKEN H. F., HAVEL R. J., GORDAN G. S., WHITTINGTON S. L. Ultracentrifugal analysis of protein-bound and free calcium in human serum. J Biol Chem. 1960 Dec;235:3654–3658. [PubMed] [Google Scholar]
  14. LUMB G. A. Determination of ionic calcium in serum. Clin Chim Acta. 1963 Jan;8:33–38. doi: 10.1016/0009-8981(63)90196-7. [DOI] [PubMed] [Google Scholar]
  15. Lewin M. R., Wills M. R., Baron D. N. Ultramicrofluorimetric determination of calcium in plasma. J Clin Pathol. 1969 Mar;22(2):222–225. doi: 10.1136/jcp.22.2.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. NATELSON S. Routine use of ultramicro methods in the clinical laboratory; estimation of sodium, potassium, chloride, protein, hematocrit value, sugar, urea and nonprotein nitrogen in fingertip blood; construction of ultramicro pipets; a practical microgasometer for estimation of carbon dioxide. Am J Clin Pathol. 1951 Dec;21(12):1153–1172. [PubMed] [Google Scholar]
  17. PRASAD A. S., FLINK E. B. Effect of carbon dioxide on concentration of calcium in an ultrafiltrate of serum obtained by centrifugation. J Appl Physiol. 1957 Jan;10(1):103–107. doi: 10.1152/jappl.1957.10.1.103. [DOI] [PubMed] [Google Scholar]
  18. Pedersen K. O. Determination of calcium fractions of serum. I. The separation of protein-bound and protein-free fractions by means of a simplified ultrafiltration technique. Scand J Clin Lab Invest. 1969 Aug;24(1):69–81. doi: 10.3109/00365516909080134. [DOI] [PubMed] [Google Scholar]
  19. Robertson W. G., Peacock M. New techniques for the separation and measurement of the calcium fractions of normal human serum. Clin Chim Acta. 1968 May;20(2):315–326. doi: 10.1016/0009-8981(68)90166-6. [DOI] [PubMed] [Google Scholar]
  20. TEREPKA A. R., TORIBARA T. Y., DEWEY P. A. The ultrafiltrable calcium of human serum. II. Variations in disease states and under experimental conditions. J Clin Invest. 1958 Jan;37(1):87–98. doi: 10.1172/JCI103589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TORIBARA T. Y., TEREPKA A. R., DEWEY P. A. The ultrafiltrable calcium of human serum. I. Ultrafiltration methods and normal values. J Clin Invest. 1957 May;36(5):738–748. doi: 10.1172/JCI103477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WALSER M. The separate effects of hyperparathyroidism, hypercalcemia of malignancy, renal failure, and acidosis on the state of calcium, phosphate, and other ions in plasma. J Clin Invest. 1962 Jul;41:1454–1471. doi: 10.1172/JCI104601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wills M. R. Biochemical consequences of chronic renal failure: a review. J Clin Pathol. 1968 Sep;21(5):541–554. doi: 10.1136/jcp.21.5.541. [DOI] [PMC free article] [PubMed] [Google Scholar]

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