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. 1993 Nov 1;90(21):10101–10104. doi: 10.1073/pnas.90.21.10101

Sequencing using pulsed field and image reconstruction.

J Noolandi 1, R Forsyth 1, A C Shi 1
PMCID: PMC47721  PMID: 8234263

Abstract

The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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