Abstract
The limits of accuracy for the quantitation of anti-D using the AutoAnalyzer have been reduced from ± 26% with manually prepared dilutions to ± 14% using an automatic pipette, as expressed by the 95% confidence limits. The error inherent in the AutoAnalyzer was estimated to contribute ± 10% to the overall error.
Problems associated with the reproducibility of this method for anti-D quantitation have been investigated, namely, the effect of the age of a given test cell, the use of different test cells, loss of sensitivity of the machine over a given day, and the reproducibility of results obtained at different positions on the standard graph.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goldsmith K. L., Mourant A. E., Bangham D. R. The International Standard for anti-Rh (anti-D) Incomplete Blood-Typing Serum. Bull World Health Organ. 1967;36(3):435–445. [PMC free article] [PubMed] [Google Scholar]
- Gunson H. H., Smith D. S. The depressant effect of the Cde(R') chromosome on the Du antigen. Vox Sang. 1967 Nov;13(5):423–430. doi: 10.1111/j.1423-0410.1967.tb03786.x. [DOI] [PubMed] [Google Scholar]
- Hughes-Jones N. C., Stevenson M. The anti-D content of IgG preparations for use in the prevention of Rh haemolytic disease. Vox Sang. 1968;14(6):401–408. doi: 10.1111/j.1423-0410.1968.tb01732.x. [DOI] [PubMed] [Google Scholar]
- Hughes-Jones N. C. The estimation of the concentration and equilibrium constant of anti-D. Immunology. 1967 May;12(5):565–571. [PMC free article] [PubMed] [Google Scholar]
- Judd W. J., Jenkins W. J. Assay of anti-D using the Technicon Auto-Analyzer and the international standard anti-D typing serum. J Clin Pathol. 1970 Dec;23(9):801–804. doi: 10.1136/jcp.23.9.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marsh W. L., Nichols M., Jenkins W. J. Automated detection of blood group antibodies. J Med Lab Technol. 1968 Oct;25(4):335–342. [PubMed] [Google Scholar]
- Moore B. P. Automation in the blood transfusion laboratory: I. Antibody dectection and quantitation in the technicon autoanalyzer. Can Med Assoc J. 1969 Feb 22;100(8):381–387. [PMC free article] [PubMed] [Google Scholar]
- NISONOFF A., PRESSMAN D. Heterogeneity and average combining constants of antibodies from individual rabbits. J Immunol. 1958 Jun;80(6):417–428. [PubMed] [Google Scholar]
- Nichols M. E., Marsh W. L. The control of enzyme solutions used in serological techniques. J Med Lab Technol. 1965 Oct;22(4):206–208. [PubMed] [Google Scholar]
- Rosenfield R. E., Szymanski I. O., Haber G. V., Kochwa S. Automated methods for the detection and measurement of hemagglutination. Bibl Haematol. 1965;23:985–991. doi: 10.1159/000384408. [DOI] [PubMed] [Google Scholar]
- Sturgeon P., Kaye B. An evaluation of the single-channel autoanalyzer in the quantitation of anti-Rh-o. Vox Sang. 1970 Jul;19(1):14–33. doi: 10.1111/j.1423-0410.1970.tb01491.x. [DOI] [PubMed] [Google Scholar]