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. 1972 Mar;25(3):206–209. doi: 10.1136/jcp.25.3.206

The adverse effect of bromelin in anti-D quantitation using the AutoAnalyzer

H H Gunson 1, P K Phillips 1,1, F Stratton 1
PMCID: PMC477263  PMID: 4622997

Abstract

With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the AutoAnalyzer for the quantitation of anti-D.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gergely J., Fudenberg H. H., van Loghem E. The papain susceptibility of IgG myeloma proteins of different heavy chain subclasses. Immunochemistry. 1970 Jan;7(1):1–6. doi: 10.1016/0019-2791(70)90025-x. [DOI] [PubMed] [Google Scholar]
  2. Goldsmith K. L., Mourant A. E., Bangham D. R. The International Standard for anti-Rh (anti-D) Incomplete Blood-Typing Serum. Bull World Health Organ. 1967;36(3):435–445. [PMC free article] [PubMed] [Google Scholar]
  3. Gunson H. H., Phillips P. K., Stratton F. Manipulative and inherent errors in anti-D quantitation using the AutoAnalyzer. J Clin Pathol. 1972 Mar;25(3):198–205. doi: 10.1136/jcp.25.3.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Judd W. J., Jenkins W. J. Assay of anti-D using the Technicon Auto-Analyzer and the international standard anti-D typing serum. J Clin Pathol. 1970 Dec;23(9):801–804. doi: 10.1136/jcp.23.9.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KISSMEYER-NIELSEN F. THE SEROLOGY OF 123 ANTI-E AND 55 ANTI-C ANTIBODIES. Sangre (Barc) 1964;10:221–224. [PubMed] [Google Scholar]
  6. Marsh W. L., Nichols M., Jenkins W. J. Automated detection of blood group antibodies. J Med Lab Technol. 1968 Oct;25(4):335–342. [PubMed] [Google Scholar]
  7. Moore B. P. Automation in the blood transfusion laboratory: I. Antibody dectection and quantitation in the technicon autoanalyzer. Can Med Assoc J. 1969 Feb 22;100(8):381–387. [PMC free article] [PubMed] [Google Scholar]
  8. Sturgeon P., Kaye B. An evaluation of the single-channel autoanalyzer in the quantitation of anti-Rh-o. Vox Sang. 1970 Jul;19(1):14–33. doi: 10.1111/j.1423-0410.1970.tb01491.x. [DOI] [PubMed] [Google Scholar]

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