There is an error in the penultimate sentence of the “Constructs” section of the Materials and Methods. The correct sentence is: For FRET experiments, the extracellular and transmembrane domains of wild-type or mutant CD4T (amino acids: 1–421), CD28 (amino acids: 1–179), and PD1 (amino acids: 1–199) were fused to mEGFP or mCherry via a short flexible linker (AAAG).
Reference
- 1.Parrish HL, Glassman CR, Keenen MM, Deshpande NR, Bronnimann MP, Kuhns MS (2015) A Transmembrane Domain GGxxG Motif in CD4 Contributes to Its Lck-Independent Function but Does Not Mediate CD4 Dimerization. PLoS ONE 10(7): e0132333 doi: 10.1371/journal.pone.0132333 [DOI] [PMC free article] [PubMed] [Google Scholar]