Abstract
During the course of some routine immunofluorescence studies `non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (`passages') in species-homologous cells subjected to serial cultivation.
Full text
PDF

Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HAYFLICK L., MOORHEAD P. S. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585–621. doi: 10.1016/0014-4827(61)90192-6. [DOI] [PubMed] [Google Scholar]
- Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
- Sander G., Hayhoe J. P., McShane A. The preparation of virus-infected cells for use in immunofluorescence techniques. J Clin Pathol. 1970 May;23(4):319–322. doi: 10.1136/jcp.23.4.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sander G. Storage of virus-infected tissue culture substrates for the detection of serum antibodies by immunofluorescence. J Clin Pathol. 1969 Nov;22(6):737–737. doi: 10.1136/jcp.22.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
