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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1972 May;25(5):441–442. doi: 10.1136/jcp.25.5.441

Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitro

G Sander 1,1, J P Jacobs 1
PMCID: PMC477343  PMID: 4114699

Abstract

During the course of some routine immunofluorescence studies `non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (`passages') in species-homologous cells subjected to serial cultivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HAYFLICK L., MOORHEAD P. S. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585–621. doi: 10.1016/0014-4827(61)90192-6. [DOI] [PubMed] [Google Scholar]
  2. Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
  3. Sander G., Hayhoe J. P., McShane A. The preparation of virus-infected cells for use in immunofluorescence techniques. J Clin Pathol. 1970 May;23(4):319–322. doi: 10.1136/jcp.23.4.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Sander G. Storage of virus-infected tissue culture substrates for the detection of serum antibodies by immunofluorescence. J Clin Pathol. 1969 Nov;22(6):737–737. doi: 10.1136/jcp.22.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]

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