Abstract
Of 129 strains of the Hong Kong variant of influenza A2 virus isolated from 176 respiratory infections, 105 (81·4%) were isolated in tissue culture, the remainder being detected only by the inoculation of fertile hen's eggs. Organ cultures of chick embryo trachea were at least as sensitive as monkey kidney tissue culture for the isolation of this virus but organ cultures of human embryonic ciliated epithelium were much less efficient. The reverse was true for strains of influenza B virus studied in 1970 and 1971 when eggs and chick embryo tracheal organ culture were of little value and organ cultures of human embryonic ciliated epithelium and monkey kidney tissue cultures were the systems of choice.
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- Butler M. Isolation and growth of mycoplasma in human embryo trachea cultures. Nature. 1969 Nov 8;224(5219):605–606. doi: 10.1038/224605a0. [DOI] [PubMed] [Google Scholar]
- Cherry J. D., Taylor-Robinson D. Large-quantity production of chicken embryo tracheal organ cultures and use in virus and mycoplasma studies. Appl Microbiol. 1970 Apr;19(4):658–662. doi: 10.1128/am.19.4.658-662.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins P. G., Ellis E. M., Woolley D. A. A comparative study of standard methods and organ culture for the isolation of respiratory viruses. J Med Microbiol. 1969 May;2(2):109–115. doi: 10.1099/00222615-2-2-109. [DOI] [PubMed] [Google Scholar]
- Roome A. P., Dickinson V., Caul E. O. Simplified organ cultures of human embryo trachea in the diagnosis of viral respiratory disease of children. J Clin Pathol. 1971 Sep;24(6):487–490. doi: 10.1136/jcp.24.6.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Votava M., Tyrrell D. A. The possible value of certain cells for the propagation of respiratory viruses. Arch Gesamte Virusforsch. 1970;29(2):253–256. doi: 10.1007/BF01249310. [DOI] [PMC free article] [PubMed] [Google Scholar]