Abstract
A relatively simple technique for sputum homogenization and dilution is described. Results show that this technique is reliable for isolation and quantitative culture of organisms from cases of chest infection. The technique has resulted in significant reduction in false positive reports, particularly when a system of interpretative reporting is utilized based on 107 organisms per millilitre of sputum being accepted as evidence of significant infection.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dixon J. M., Miller D. C. Value of dilute inocula in cultural examination of sputum. Lancet. 1965 Nov 20;2(7421):1046–1048. doi: 10.1016/s0140-6736(65)90572-6. [DOI] [PubMed] [Google Scholar]
- KASS E. H. Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic. AMA Arch Intern Med. 1957 Nov;100(5):709–714. doi: 10.1001/archinte.1957.00260110025004. [DOI] [PubMed] [Google Scholar]
- Monroe P. W., Muchmore H. G., Felton F. G., Pirtle J. K. Quantitation of microorganisms in sputum. Appl Microbiol. 1969 Aug;18(2):214–220. doi: 10.1128/am.18.2.214-220.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pirtle J. K., Monroe P. W., Smalley T. K., Mohr J. A., Rhoades E. R. Diagnostic and therapeutic advantages of serial quantitative cultures of fresh sputum in acute bacterial pneumonia. Am Rev Respir Dis. 1969 Dec;100(6):831–838. doi: 10.1164/arrd.1969.100.6.831. [DOI] [PubMed] [Google Scholar]