Skip to main content
. 2016 Feb 23;5:e09462. doi: 10.7554/eLife.09462
Reagent Type Manufacturer Catalog # Comments
PC9 Cells Human cell line Sigma-Aldrich 90071810 Originally obtained from
Dr. Kazuto Nishio (National
Cancer Center Hospital,
Tokyo)
PC9 DTP cells Human cell line n/a n/a Generated according
to protocol 2
10 cm dishes Labware Corning 430167 Original brand not specified.
RPMI 1640 (4.5 g/l glucose) Cell culture ATCC 30-2001 Original brand
not specified
Fetal bovine serum (FBS) Cell culture HyClone SH30910.03 Original brand
not specified
Penicillin-Streptomycin
(5,000 U/ml)
Cell culture Life Technologies 15070-063 Not originally reported.
RIPA buffer Buffer Sigma-Aldrich R0278 From replicating
lab protocol
Complete mini protease
inhibitor cocktail tablets
Inhibitor Roche Diagnostics 11 836 153 001 From replicating
lab protocol
Halt phosphatase
inhibitor cocktail
Inhibitor Thermo Scientific 1862495 From replicating
lab protocol
Phenylmethanesulfonyl
fluoride solution
Inhibitor Sigma-Aldrich 93482
Pierce BCA Protein
Assay Kit
Protein Assay Thermo Fisher 23225 From replicating
lab protocol
NuPAGE LDS sample
buffer (4X)
Western blot
reagent
Life Technologies NP0007 Replaces Laemmli
sample buffer
Molecular weight markers Western blot
reagent
Li-Cor 928-40000 Not originally reported.
NuPage Sample Reducing
Agent (10X)
Western blot
reagent
Life Technologies NP0004 From replicating
lab protocol
NuPAGE 4-12%
Bis-Tris gels
(10 well/15 well)
Western blot
reagent
Life Technologies NP0335BOX/
NP0336BOX
From replicating
lab protocol
NuPAGE MES SDS
Running Buffer (20X)
Western blot
reagent
Life Technologies NP0002 From replicating
lab protocol
NuPAGE MOPS
SDS Running
Buffer (20X)
Western blot
reagent
Life Technologies NP0001 From replicating
lab protocol
iBlot gel transfer
stacks
nitrocellulose
Western blot
reagent
Life Technologies IB301002 From replicating lab protocol
Rabbit anti-H3K14Ac
antibody
Antibodies Active Motif 39698 Original catalog number
not specified. Note that
each new batch is
associated with a new
catalog number.
Rabbit anti-GAPDH
antibody
Antibodies Life Technologies PA1-988 Original was from Biosource
International (acquired by Life
Technologies)
1:500-1:5000
suggested dilution
Mouse anti-GAPDH
antibody (clone GA1R)
Antibodies Life Technologies MA5-15738 Original was from Biosource
International (acquired by
Life Technologies)
1:1000-
1:10,000 suggested dilution
Mouse anti-H3
antibody (clone 1B1-B2)
Antibodies Active Motif 61476 Original catalog number not
specified. Note that each new batch
is associated with a new
catalog number.
Mouse anti-CD133
antibody
(clone 17A6.1)
Antibodies EMD Millipore MAB4399 Replaces Cell Signaling
Technology brand (discontinued)
Rabbit anti-pEGFR (Y1068)
antibody (clone D7A5)
Antibodies Cell Signaling Technology 3777 Original catalog number not specified.
Mouse anti-EGFR
antibody
(clone 1F4)
Antibodies Cell Signaling Technology 2239 Replaces Santa Cruz brand.
Donkey anti-mouse
IRDye 680RD
Antibodies Li-Cor 926-68072 Replaces HRP
conjugated
antibodies
Donkey anti-rabbit
IRDye 800CW
Antibodies Li-Cor 926-32213
Odyssey Infrared
Imager
Instrument Li-Cor Biosciences CLx
Image Studio Software Li-Cor Biosciences