Reagent | Type | Manufacturer | Catalog # | Comments |
---|---|---|---|---|
PC9 Cells | Human cell line | Sigma-Aldrich | 90071810 | Originally obtained from Dr. Kazuto Nishio (National Cancer Center Hospital, Tokyo) |
PC9 DTP cells | Human cell line | n/a | n/a | Generated according to protocol 2 |
10 cm dishes | Labware | Corning | 430167 | Original brand not specified. |
RPMI 1640 (4.5 g/l glucose) | Cell culture | ATCC | 30-2001 | Original brand not specified |
Fetal bovine serum (FBS) | Cell culture | HyClone | SH30910.03 | Original brand not specified |
Penicillin-Streptomycin (5,000 U/ml) |
Cell culture | Life Technologies | 15070-063 | Not originally reported. |
RIPA buffer | Buffer | Sigma-Aldrich | R0278 | From replicating lab protocol |
Complete mini protease inhibitor cocktail tablets |
Inhibitor | Roche Diagnostics | 11 836 153 001 | From replicating lab protocol |
Halt phosphatase inhibitor cocktail |
Inhibitor | Thermo Scientific | 1862495 | From replicating lab protocol |
Phenylmethanesulfonyl fluoride solution |
Inhibitor | Sigma-Aldrich | 93482 | |
Pierce BCA Protein Assay Kit |
Protein Assay | Thermo Fisher | 23225 | From replicating lab protocol |
NuPAGE LDS sample buffer (4X) |
Western blot reagent |
Life Technologies | NP0007 | Replaces Laemmli sample buffer |
Molecular weight markers | Western blot reagent |
Li-Cor | 928-40000 | Not originally reported. |
NuPage Sample Reducing Agent (10X) |
Western blot reagent |
Life Technologies | NP0004 | From replicating lab protocol |
NuPAGE 4-12% Bis-Tris gels (10 well/15 well) |
Western blot reagent |
Life Technologies | NP0335BOX/ NP0336BOX |
From replicating lab protocol |
NuPAGE MES SDS Running Buffer (20X) |
Western blot reagent |
Life Technologies | NP0002 | From replicating lab protocol |
NuPAGE MOPS SDS Running Buffer (20X) |
Western blot reagent |
Life Technologies | NP0001 | From replicating lab protocol |
iBlot gel transfer stacks nitrocellulose |
Western blot reagent |
Life Technologies | IB301002 | From replicating lab protocol |
Rabbit anti-H3K14Ac antibody |
Antibodies | Active Motif | 39698 | Original catalog number not specified. Note that each new batch is associated with a new catalog number. |
Rabbit anti-GAPDH antibody |
Antibodies | Life Technologies | PA1-988 | Original was from Biosource International (acquired by Life Technologies) 1:500-1:5000 suggested dilution |
Mouse anti-GAPDH antibody (clone GA1R) |
Antibodies | Life Technologies | MA5-15738 | Original was from Biosource International (acquired by Life Technologies) 1:1000- 1:10,000 suggested dilution |
Mouse anti-H3 antibody (clone 1B1-B2) |
Antibodies | Active Motif | 61476 | Original catalog number not specified. Note that each new batch is associated with a new catalog number. |
Mouse anti-CD133 antibody (clone 17A6.1) |
Antibodies | EMD Millipore | MAB4399 | Replaces Cell Signaling Technology brand (discontinued) |
Rabbit anti-pEGFR (Y1068) antibody (clone D7A5) |
Antibodies | Cell Signaling Technology | 3777 | Original catalog number not specified. |
Mouse anti-EGFR antibody (clone 1F4) |
Antibodies | Cell Signaling Technology | 2239 | Replaces Santa Cruz brand. |
Donkey anti-mouse IRDye 680RD |
Antibodies | Li-Cor | 926-68072 | Replaces HRP conjugated antibodies |
Donkey anti-rabbit IRDye 800CW |
Antibodies | Li-Cor | 926-32213 | |
Odyssey Infrared Imager |
Instrument | Li-Cor Biosciences | CLx | |
Image Studio | Software | Li-Cor Biosciences |