Figure 4. The concentration and identity of each Ras network component can modulate the timing, duration, shape, or amplitude of effector outputs.
(A) Depiction of the experimental setup: a fixed step-input is applied to a panel of Ras signaling systems in which the concentration of a single network component is varied to determine how each network component individually modulates system output. (B) Absolute and normalized effector responses to step-input in the presence of increasing amounts of the NF1 gap. (C) Absolute and normalized effector responses to step-input in the presence of increasing amounts of the p120 GAP. (D) Absolute and normalized responses to step-input in the presence of different densities of Ras on the bead surface. (E) Absolute and normalized responses to step-input in the presence of increasing amounts of the C-Raf RBD effector. GAP, GTPase-activating protein; RBD, Ras-binding domain.