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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Clin Infect Dis. 2015 Dec 17;62(6):774–776. doi: 10.1093/cid/civ1021

Use of the Novel Therapeutic Agent Miltefosine for the Treatment of Primary Amebic Meningoencephalitis: Report of One Fatal and One Surviving Case

Jennifer R Cope 1, Dennis A Conrad 2, Naiomi Cohen 3, Manuel Cotilla 4, Alexandre DaSilva 5, Jonathan Jackson 6, Govinda Visvesvara 7
PMCID: PMC4775347  NIHMSID: NIHMS753758  PMID: 26679626

Abstract

Primary amebic meningoencephalitis (PAM) is a fulminant central nervous system infection caused by the thermophilic free-living ameba Naegleria fowleri. Few survivals have been documented and adequate treatment is lacking. We report two PAM cases, one fatal and one surviving, treated with the novel antiparasitic agent miltefosine.

Keywords: primary amebic meningoencephalitis, Naegleria fowleri, miltefosine


Primary amebic meningoencephalitis (PAM) is a fulminant central nervous system infection caused by the thermophilic free-living ameba Naegleria fowleri. The infection occurs when freshwater containing the ameba enters the nose, crosses the cribriform plate, and enters the brain. PAM is often clinically indistinguishable from bacterial meningitis, with early symptoms of headache, fever, nausea, and vomiting progressing rapidly to altered mental status, seizures, coma, and death [1]. Despite the availability of several antimicrobial agents that show in vitro activity against Naegleria fowleri, use of these agents clinically, even when administered early in the course of illness, has resulted in few survivors. Amphotericin B has been the mainstay of PAM treatment and all of the well-documented survivors have received it as part of their treatment regimen [25]. The antiparasitic agent miltefosine has shown some promise for the treatment of free-living ameba infections [6].

Case report 1

A 12-year-old male presented to a local community hospital on August 7, 2013 with a one-day history of headaches, weakness, vomiting, fevers (39.4 °C), and altered mental status. The patient lived in a wooded area of central Florida where, on August 5, he was riding on a kneeboard pulled by an all-terrain vehicle with friends in a 2–3 foot deep ditch filled with stagnant rainwater. While participating in this activity, his head was, at times, completely submerged underwater. Lumbar puncture was performed with an opening pressure of 50 cm H2O (normal 10–20 cm H2O). Cerebrospinal fluid was noted to be cloudy with a white blood cell (WBC) count of 10,216 cells/μL, a red blood cell (RBC) count of 3,500 cells/μL, protein 560 mg/dL, and glucose <20 mg/dL. Computed tomography (CT) of the head without contrast was normal. Broad antimicrobial coverage was started and included acyclovir, liposomal amphotericin B, fluconazole, rifampin, vancomycin, and ceftriaxone. The following day, the patient’s neurologic status deteriorated. In consultation with the Centers for Disease Control and Prevention (CDC), the treatment regimen was tailored to include deoxycholate amphotericin B intravenously, fluconazole, azithromycin, and rifampin. Miltefosine was added once it arrived approximately 31 hours after his initial presentation. The diagnosis of PAM was confirmed with the identification of Naegleria fowleri in the CSF by polymerase chain reaction (PCR) testing at CDC, at which time a lumbar drain was placed and intrathecal amphotericin B was added. A follow-up CT demonstrated cerebral edema which was managed with hypertonic saline, mannitol, surgical decompression, and therapeutic hypothermia with no clinical improvement. Subsequent imaging showed brain herniation and the patient was declared brain dead on hospital day 16.

Case report 2

On August 17, 2013, an 8-year-old Hispanic male presented to a Texas hospital with a five day history of fever, headache, chills, nausea, and vomiting progressing to photophobia and altered mental status. The patient’s mother had sought medical care at three clinics in Mexico prior to seeking care in the United States. The patient had been spending the summer with his mother, who lived in an informal settlement adjacent to the Rio Grande. This settlement had no potable public water supply or sanitary sewer system; water for consumption was purchased in Texas, whereas water used for bathing and cleaning was obtained by direct piping of surface water from the Rio Grande. The patient enjoyed “splashing in the shallows” at the edge of the river. CSF appeared cloudy with a RBC count of 1,000 cells/μL, a WBC count of 2,312 cells/μL with 92% neutrophils, and protein 311 mg/dL. The glucose concentration could not be determined accurately. When the CSF Gram stain did not show any organisms, a Wright stain was performed revealing amebic trophozoites.

With a Glasgow Coma Score of 3, the patient was intubated and mechanically ventilated. A right frontal external ventricular drainage (EVD) catheter was placed in order to monitor intracranial pressure and provide therapeutic drainage as warranted. At the time of placement, the intraventricular pressure exceeded 40 mm Hg (normal 1–20 mm Hg). Treatment for PAM was promptly initiated and is summarized in Table 1. Miltefosine was requested from CDC and was administered 14 hours after the patient was admitted to the PICU. At the conclusion of his treatment course, he could react with healthcare providers only by withdrawal from noxious stimuli. He was able to breathe spontaneously, but did not have a consistent gag or cough reflex, nor could he evidence any coordinated ability for self-care activities. After a 39-day hospital stay, the patient spent another 36 days on the pediatric rehabilitation service. He was discharged to home in the care of his family. Approximately eighteen months following discharge, the patient has static encephalopathy with profound persistent mental disability, seizure disorder partially controlled with anticonvulsant therapy, is non-verbal and cannot care for himself.

Table 1.

PAM Survivor Treatment and Outcomes

Australian Survivor (1971)
[2]
U.S. Survivor (1978)
[3]
Mexican Survivor (2003)
[4]
U.S. Female Survivor (2013)
[5]
U.S. Male Survivor (2013)
[current case]
Symptom onset to initiation of anti-ameba therapy (hours) Unknown >72 9 48 >120
Anti-ameba drug therapies Amphotericin B (dose unknown) IV, IT, and via ventricular reservoir Amphotericin B 1–1.5 mg/kg/day IV in 2 divided doses × 9 days; 1–1.5 mg IT daily × 10 days Amphotericin B 0.25–1 mg/kg/day IV × 14 days Amphotericin B 1–1.5 mg/kg/day IV in 2 divided doses × 26 days; 1–1.5 mg IT daily × 10 days Amphotericin B 1 mg/kg/day IV × 19 days; 0.1 mg IT × 5 days
Rifampin 10 mg/kg/day PO in 3 divided doses × 9 days Rifampin 10 mg/kg/day PO × 1 month Rifampin 10 mg/kg/day IV × 26 days Rifampin 12 mg/kg/day PO × 19 days
Miconazole 350 mg/m2 body surface area/day IV in 3 divided doses × 9 days
10 mg daily then 10 mg IT every other day × 8 days
Fluconazole 10 mg/kg/day IV (then PO) × 1 month Fluconazole 10 mg/kg/day IV × 26 days Fluconazole 12 mg/kg/day loading dose, then 9 mg/kg/day IV × 19 days
Azithromycin 10 mg/kg/day IV × 26 days Azithromycin 10 mg/kg/day PO × 19 days
Miltefosine 150 mg PO in 3 divided doses × 26 days Miltefosine 150 mg PO in 3 divided doses × 19 days
Adjunctive therapies Dexamethasone Dexamethasone 0.6 mg/kg/day IV Dexamethasone 0.6 mg/kg/day IV Dexamethasone 0.6 mg/kg/day IV
CSF drainage CSF drainage
Hyperosmolar therapy (mannitol and 3% saline) Hyperosmolar therapy (mannitol)
Moderate hyperventilation
Induced hypothermia (32–34°C)
Outcome Complete recovery Complete recovery Complete recovery Complete recovery Significant neurologic deficits

IV=intravenous; IT=intrathecal; PO=per oral

Discussion

These two PAM patients are notable in that they received the novel antiparasitic agent miltefosine as part of their treatment regimen. Miltefosine is an alkylphosphocholine compound that has predominantly been studied for the treatment of leishmaniasis [7]. Its antileishmanial and antiamebic mechanisms of action are unknown. The surviving patient reported here is the fifth case of well-documented survival in a PAM infection (Table 1). During the same summer in which these two PAM cases were diagnosed, a 12-year old adolescent female from Arkansas, USA was diagnosed with PAM and survived, making a full neurologic recovery [5]. Her treatment regimen included all of the drugs given to the two patients reported here. Notable differences in her clinical course and treatment from the two patients reported here included being treated with Naegleria-specific drugs approximately 48 hours after symptom onset and undergoing aggressive management of elevated intracranial pressure, including therapeutic hypothermia. In contrast, the 8-year-old Texas male survivor reported here had symptoms for five days before a PAM diagnosis was made and specific PAM therapy was initiated. While his elevated intracranial pressure was managed both medically and surgically, therapeutic hypothermia was not used in his case. The Florida child presented here did present early in the course of illness. However, some aspects of his care differed slightly from the Arkansas survivor, including having a lumbar drain placed (vs. EVD), receiving intrathecal amphotericin over two days after presentation to the hospital, and initially receiving liposomal amphotericin B (in vitro testing shows deoxycholate amphotericin B has lower MICs for Naegleria fowleri). However, it us unknown whether these small differences in care contributed to the varied outcomes of these three patients.

All three patients (the two reported here plus the Arkansas survivor) received miltefosine as part of their treatment regimen but with three very different outcomes: death, survival with poor neurologic outcome, and survival with full neurologic recovery. Therefore, while miltefosine is a promising anti-Naegleria agent, it is not a magic bullet, and its administration does not assure recovery. Since 2013, when miltefosine became available through CDC, 100% (2/2) of surviving U.S. PAM cases received miltefosine compared with 33% (3/9) of fatal U.S. PAM cases (CDC unpublished data). Surviving PAM, including survival with few or no deficits is likely multifactorial and includes early diagnosis and treatment, using combination drug therapy (including miltefosine), and aggressive management of elevated intracranial pressure based on the principles of traumatic brain injury [5].

Between 1962 and 2014, 133 cases of PAM were reported to CDC, with the majority occurring in southern-tier states in patients with recent exposure to warm freshwater lakes and rivers [1]. While the number of infections reported annually has remained stable (0–8), recent changes in the epidemiology of PAM are concerning. For the first time in 2010, a PAM case was reported from the northern state of Minnesota followed by additional cases from Minnesota, Indiana, and Kansas in 2011 and 2012, raising concerns about an expanding geographic range of illness caused by this thermophilic, potentially climate-sensitive, pathogen [1]. In addition to exposure to recreational water, nasal exposure to tap water has now been associated with several PAM cases, including two patients who used tap water in a neti pot to irrigate their sinuses, one patient who used tap water to perform ritual ablution, and one patient who played on a backyard water slide supplied with tap water [810]. Given these changes, clinicians in all regions of the United States should consider the diagnosis of PAM in a patient with meningitis and recent nasal freshwater exposure.

Clinicians who suspect PAM in a patient under their care should contact the CDC Emergency Operations Center (770-488-7100) for 24/7 diagnostic assistance, specimen collection guidance, and treatment recommendations, including the use of miltefosine. Confirmatory PCR testing is available at CDC with a turnaround time of 2–4 hours. Miltefosine is available directly from CDC under an expanded access investigational new drug (IND) protocol for treatment of free-living ameba infections in the United States [11]. With increased awareness of this deadly infection, prompt diagnosis and initiation of combination drug therapy, and aggressive management of elevated intracranial pressure, more patients might survive PAM. Clinicians are encouraged to report all suspected PAM cases to CDC, as each case provides valuable information for improving understanding of pathogenesis and treatment, regardless of outcome.

Acknowledgments

The authors wish to acknowledge Almea Matanock, MD, Jamae Morris, PhD, and the staff of CDC’s Drug Service and Emergency Operations Center for their roles in facilitating the rapid release of miltefosine from CDC for the treatment of these two patients.

Financial support. This work was conducted as part of the authors’ regular job duties.

Footnotes

Potential conflict of interest. All authors: No reported conflicts.

The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.

Contributor Information

Jennifer R. Cope, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Dennis A. Conrad, University of Texas Health Science Center, San Antonio, TX, USA.

Naiomi Cohen, Miami Children’s Hospital, Miami, FL, USA.

Manuel Cotilla, Miami Children’s Hospital, Miami, FL, USA.

Alexandre DaSilva, U.S. Food and Drug Administration, Laurel, MD, and Centers for Disease Control and Prevention, Atlanta, GA, USA.

Jonathan Jackson, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Govinda Visvesvara, Centers for Disease Control and Prevention, Atlanta, GA, USA.

References

  • 1.Capewell LGHA, Yoder JS, Cope JR, et al. Diagnosis, clinical course, and treatment of primary amoebic meningoencephalitis in the United States, 1937–2013. J Ped Infect Dis. 2014:1–8. doi: 10.1093/jpids/piu103. [DOI] [PubMed] [Google Scholar]
  • 2.Anderson K, Jamieson A. Primary amoebic meningoencephalitis. Lancet. 1972;2:379. doi: 10.1016/s0140-6736(72)91763-1. [DOI] [PubMed] [Google Scholar]
  • 3.Seidel JS, Harmatz P, Visvesvara GS, Cohen A, Edwards J, Turner J. Successful treatment of primary amebic meningoencephalitis. N Engl J Med. 1982;306:346–8. doi: 10.1056/NEJM198202113060607. [DOI] [PubMed] [Google Scholar]
  • 4.Vargas-Zepeda J, Gomez-Alcala AV, Vasquez-Morales JA, Licea-Amaya L, De Jonckheere JF, Lares-Villa F. Successful treatment of Naegleria fowleri meningoencephalitis by using intravenous amphotericin B, fluconazole and rifampicin. Arch Med Res. 2005;36:83–6. doi: 10.1016/j.arcmed.2004.11.003. [DOI] [PubMed] [Google Scholar]
  • 5.Linam WM, Ahmed M, Cope JR, et al. Successful treatment of an adolescent with Naegleria fowleri primary amebic meningoencephalitis. Pediatrics. 2015;135:e744–8. doi: 10.1542/peds.2014-2292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Martinez DY, Seas C, Bravo F, et al. Successful treatment of Balamuthia mandrillaris amoebic infection with extensive neurological and cutaneous involvement. Clin Infect Dis. 2010;51:e7–11. doi: 10.1086/653609. [DOI] [PubMed] [Google Scholar]
  • 7.Sundar SS, Jha TK, Thakur CP, et al. Oral miltefosine for Indian visceral leishmaniasis. N Engl J Med. 2002;347:1739–46. doi: 10.1056/NEJMoa021556. [DOI] [PubMed] [Google Scholar]
  • 8.Yoder JS, Straif-Bourgeois S, Roy SL, et al. Primary amebic meningoencephalitis deaths associated with sinus irrigation using contaminated tap water. Clin Infect Dis. 2012;55:e79–85. doi: 10.1093/cid/cis626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Centers for Disease Control and Prevention. Notes from the field: primary amebic meningoencephalitis associated with ritual nasal rinsing--St. Thomas, U.S Virgin islands, 2012. MMWR Morb Mortal Wkly Rep. 2013;62:903. [PMC free article] [PubMed] [Google Scholar]
  • 10.Cope JR, Ratard RC, Hill VR, et al. The first association of a primary amebic meningoencephalitis death with culturable Naegleria fowleri in tap water from a US treated public drinking water system. Clin Infect Dis. 2015;60:e36–42. doi: 10.1093/cid/civ017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Centers for Disease Control and Prevention. Investigational drug available directly from CDC for the treatment of infections with free-living amebae. MMWR Morb Mortal Wkly Rep. 2013;62:666. [PMC free article] [PubMed] [Google Scholar]

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