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. 2016 Feb 15;6(4):545–557. doi: 10.7150/thno.13352

Figure 5.

Figure 5

NAP9 induces cardiac protection in mice subjected to IR. A. LVEF values from non ischemic (healthy) mice and from mice who underwent IR for 24 hours and were injected with 50 mg/Kg NAP9, NAPSC, or saline (Control) right after ischemia (n=9 mice/group. Mean ± SD; *p <0.05, NAP9 vs Control). B. Measurement of LV necrotic area as percentage with respect to the area at risk, detected by double Evans Blue/TTC staining (n = 18 mice/group; mean ± SD; *p <0.05, NAP9 vs Control). C. Immunoblot detection of MMP9 (upper panel), and MMP-2 (lower panel) in non ischemic (Healthy) mice, and in mice injected with 50 mg/Kg NAP9, NAPSC, or saline (Control) right after reperfusion. GAPDH was used as loading control (n=9 mice/group; mean ± SD; *p <0.05, NAP9 vs Control and NAPSC). D. Detection of MMP-9 by Gelatin zymography in the same hearts as in C. The two bands of 92 kDa and 87kDa correspond with the sizes of proMMP-9 and active-MMP-9 respectively. LMW: low molecular weight. HMG: high molecular weight (n=9 mice/group; mean ± SD; *p <0.05, NAP9 vs Control and NAPSC).