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Retention of humanized LAT-27 in NOG mice. Each of three NOG mice was infused i.v. with 1 mg/head hu-LAT-27, and the LAT-27 concentrations in their sera were measured daily by ELISA using gp46 peptide (180–204) as antigen and secondary anti-human IgG-HRP, in which purified hu-LAT-27 was used to draw a standard curve.
| Days after Injection | hu-LAT-27 Concentration in Serum (μg/mL) | ||
|---|---|---|---|
| NOG#1 | NOG#2 | NOG#3 | |
| 1 | 268 | 249 | 277 |
| 2 | 114 | 155 | 124 |
| 3 | 79 | 88 | 80 |
| 4 | 57 | 86 | 61 |
| 5 | 49 | 47 | 48 |
