Abstract
The localization of Australia antigen in the liver by the fluorescent antibody technique (direct method) has been studied. Specific fluorescence was observed in the cytoplasm of liver cells. Two distribution patterns of fluorescent-positive cells, namely, a diffuse fluorescence in the liver lobules and a spotty distribution of fluorescent-positive cells, were observed.
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- Ahmed M. N., Huang S., Spence L. Australia antigen and hepatitis. An electron microscopic study. Arch Pathol. 1971 Jul;92(1):66–72. [PubMed] [Google Scholar]
- CURTAIN C. C. The chromatographic purification of fluorescein-antibody. J Histochem Cytochem. 1961 Sep;9:484–486. doi: 10.1177/9.5.484. [DOI] [PubMed] [Google Scholar]
- Coyne V. E., Millman I., Cerda J., Gerstley B. J., London T., Sutnick A., Blumberg B. S. The localization of Australia antigen by immunofluorescence. J Exp Med. 1970 Feb;131(2):307–319. doi: 10.1084/jem.131.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edgington T. S., Ritt D. J. Intrahepatic expression of serum hepatitis virus-associated antigens. J Exp Med. 1971 Oct 1;134(4):871–885. doi: 10.1084/jem.134.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOLDSTEIN G., SLIZYS I. S., CHASE M. W. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins. J Exp Med. 1961 Jul 1;114:89–110. doi: 10.1084/jem.114.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
- MCKINNEY R. M., SPILLANE J. T., PEARCE G. W. FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS. J Immunol. 1964 Aug;93:232–242. [PubMed] [Google Scholar]
- Nielsen J. O., Elling P. Investigations of liver biopsies for Australia antigen by immunofluorescent technique. Clin Exp Immunol. 1971 Dec;9(6):699–705. [PMC free article] [PubMed] [Google Scholar]
- Nowoslawski A., Brzosko W. J., Madaliński K., Krawczyński K. Cellular localisation of Australia antigen in the liver of patients with lymphoproliferative disorders. Lancet. 1970 Mar 7;1(7645):494–498. doi: 10.1016/s0140-6736(70)91580-1. [DOI] [PubMed] [Google Scholar]
- Scotto J., Homberg J. C., Stralin H., Salmon C., Caroli J. Observation ultrastructurale d'inclusions cytoplasmiques particulières dans des cas d'hépatites virales. Rapport avec l'antigène austrialia? Presse Med. 1970 Nov 7;78(47):2061–2064. [PubMed] [Google Scholar]
- Stein O., Fainaru M., Stein Y. Virus-like particles in cytoplasm of livers of Au-antigen carriers. Lancet. 1971 Jul 10;2(7715):90–91. doi: 10.1016/s0140-6736(71)92060-5. [DOI] [PubMed] [Google Scholar]




