Abstract
A microculture technique is described in which human peripheral blood leucocytes undergo DNA synthesis in response to specific and non-specific blastogenic factors. A sensitive Millipore filter assay technique is shown to be suitable for determining the incorporation of radioactive thymidine into the DNA of the stimulated cells in these microcultures. With these methods it is possible to measure the DNA synthesis in a 50-μl culture containing an innoculum of only 105 leucocytes. This small culture volume, with its low number of leucocytes, therefore makes it possible to perform experiments when either the available leucocytes or blastogenic factors are in limited supply.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Robbins J. H., Burk P. G., Levis W. R. Rapid and sensitive assay for D.N.A. synthesis in leucocyte cultures. Lancet. 1970 Jul 11;1(7663):98–98. doi: 10.1016/s0140-6736(70)92667-x. [DOI] [PubMed] [Google Scholar]
- Robbins J. H., Gart J. J., Levis W. R., Burk P. G. The millipore filter assay technique for measuring tritiated thymidine incorporation into DNA in leucocyte cultures. Clin Exp Immunol. 1972 Aug;11(4):629–640. [PMC free article] [PubMed] [Google Scholar]