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. 1973 Jan;26(1):37–41. doi: 10.1136/jcp.26.1.37

The separation of epinephrine from norepinephrine and dopamine from DOPA on Sephadex G-10

T J McDonald 1,2, B E P Murphy 1,2
PMCID: PMC477646  PMID: 4693897

Abstract

Epinephrine and norepinephrine were separated by acid elution through a Sephadex G-10 column with high recovery (better than 90%), excellent reproducibility, and little overlap (less than 10%). Once packed, the columns could be re-used indefinitely. Total elution time was about six hours and the columns could be left untended since the gravity flow stops automatically once the level of the eluant reaches the gel bed. The resulting dilution was five-fold. 3,4-Dihydroxyphenylalanine (DOPA) was completely separated from 3,4-dihydroxyphenylethylamine (dopamine) by the same procedure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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