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. 1973 Apr;26(4):268–272. doi: 10.1136/jcp.26.4.268

Indirect cutaneous immunofluorescence

II Clinical significance

Thomas K Burnham 1
PMCID: PMC477703  PMID: 4573789

Abstract

Sera of 532 patients with bullous diseases, connective tissue diseases and malignancies were tested for pemphigus epidermal intercellular fluorescence (ICF) and for the bullous pemphigoid `tubular' band by the indirect fluorescent antibody technique. Human normal skin cryostat sections were used.

The band and ICF were seen primarily only in bullous pemphigoid and pemphigus respectively, Some indirect band and ICF-negative patients demonstrated positive direct results in involved skin. suggesting that direct tests should be performed in indirect negative patients clinically thought to have pemphigus or bullous pemphigoid.

No close correlation was found between disease activity and positive or negative indirect tests in bullous pemphigoid and pemphigus. Steroids did not interfere with positive results of this diagnostically extremely valuable test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEUTNER E. H., JORDON R. E. DEMONSTRATION OF SKIN ANTIBODIES IN SERA OF PEMPHIGUS VULGARIS PATIENTS BY INDIRECT IMMUNOFLUORESCENT STAINING. Proc Soc Exp Biol Med. 1964 Nov;117:505–510. doi: 10.3181/00379727-117-29622. [DOI] [PubMed] [Google Scholar]
  2. BEUTNER E. H., LEVER W. F., WITEBSKY E., JORDON R., CHERTOCK B. AUTOANTIBODIES IN PEMPHIGUS VULGARIS: RESPONSE TO AN INTERCELLULAR SUBSTANCE OF EPIDERMIS. JAMA. 1965 May 24;192:682–688. doi: 10.1001/jama.1965.03080210026006. [DOI] [PubMed] [Google Scholar]
  3. BURNHAM T. K., NEBLETT T. R., FINE G. THE APPLICATION OF THE FLUORESCENT ANTIBODY TECHNIC TO THE INVESTIGATION OF LUPUS ERYTHEMATOSUS AND VARIOUS DERMATOSES. J Invest Dermatol. 1963 Dec;41:451–456. doi: 10.1038/jid.1963.140. [DOI] [PubMed] [Google Scholar]
  4. Bean S. F., Good R. A., Windhorst D. B. Bullous pemphigoid in an 11-year-old boy. Arch Dermatol. 1970 Aug;102(2):205–208. [PubMed] [Google Scholar]
  5. Beutner E. H., Chorzelski T. P., Hale W. L., Hausmanowa-Petrusewicz I. Autoimmunity in concurrent myasthenia gravis and pemphigus erythematosus. JAMA. 1968 Mar 4;203(10):845–849. [PubMed] [Google Scholar]
  6. Beutner E. H., Jordon R. E., Chorzelski T. P. The immunopathology of pemphigus and bullous pemphigoid. J Invest Dermatol. 1968 Aug;51(2):63–80. [PubMed] [Google Scholar]
  7. Burnham T. K., Fine G. Indirect cutaneous immunofluorescence. I. Morphologic observations in bullous diseases, malignancies, and connective tissue diseases. Arch Dermatol. 1972 Jan;105(1):52–58. doi: 10.1001/archderm.105.1.52. [DOI] [PubMed] [Google Scholar]
  8. Burnham T. K., Fine G. The immunofluorescent "band" test for lupus erythematosus. 3. Employing clinically normal skin. Arch Dermatol. 1971 Jan;103(1):24–32. [PubMed] [Google Scholar]
  9. Burnham T. K., Neblett T. R., Fine G., Bank P. The immunofluorescent tumor imprint technique. IV. The significance of "thready" nuclear immunofluorescence. Arch Dermatol. 1969 May;99(5):611–616. [PubMed] [Google Scholar]
  10. Burnham T. K., Neblett T. R., Fine G. Immunofluorescent "band" test for lupus erythematosus. II. Employing skin lesions. Arch Dermatol. 1970 Jul;102(1):42–50. [PubMed] [Google Scholar]
  11. Burnham T. K., Neblett T. R., Fine G. The immunofluorescent tumor imprint technic. I. Description and evaluation. Am J Clin Pathol. 1966 Jun;45(6):714–721. doi: 10.1093/ajcp/45.6.714. [DOI] [PubMed] [Google Scholar]
  12. Chorzelski T. P., Von Weiss J. F., Lever W. F. Clinical significance of autoantibodies in pemphigus. Arch Dermatol. 1966 May;93(5):570–576. [PubMed] [Google Scholar]
  13. Chorzelski T., Jablońska S., Blaszczyk M., Jarzabek M. Autoantibodies in pemphigoid. Dermatologica. 1968;136(5):325–334. doi: 10.1159/000254118. [DOI] [PubMed] [Google Scholar]
  14. Fiumara N. J. A laboratory test is not a diagnosis. JAMA. 1971 Jul 5;217(1):71–71. [PubMed] [Google Scholar]
  15. Jordon R. E., Beutner E. H., Witebsky E., Blumental G., Hale W. L., Lever W. F. Basement zone antibodies in bullous pemphigoid. JAMA. 1967 May 29;200(9):751–756. [PubMed] [Google Scholar]
  16. Jordon R. E., Sams W. M., Jr, Beutner E. H. Complement immunofluorescent staining in bullous pemphigoid. J Lab Clin Med. 1969 Oct;74(4):548–556. [PubMed] [Google Scholar]
  17. Katz S. I., Halprin K. M., Inderbitzin T. M. The use of human skin for the detection of anti-epithelial autoantibodies. A diagnostic and prognostic test. J Invest Dermatol. 1969 Dec;53(6):390–399. doi: 10.1038/jid.1969.166. [DOI] [PubMed] [Google Scholar]
  18. Sams W. M., Jr Bullous pemphigoid. Is it an immunologic disease? Arch Dermatol. 1970 Nov;102(5):485–497. doi: 10.1001/archderm.102.5.485. [DOI] [PubMed] [Google Scholar]
  19. Schroeter A., Sams W. M., Jr, Jorden R. E. Immunofluorescent studies of pemphigus foliaceus in a child. Arch Dermatol. 1969 Dec;100(6):736–740. [PubMed] [Google Scholar]

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