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. 1973 May;26(5):354–358. doi: 10.1136/jcp.26.5.354

Effect of intravenous prostaglandin E2 on platelet function, coagulation, and fibrinolysis

P W Howie 1,2, A A Calder 1,2, C D Forbes 1,2, C R M Prentice 1,2
PMCID: PMC477734  PMID: 4714960

Abstract

Prostaglandin E2 (PGE2) was infused intravenously to eight women for the termination of pregnancy and tests of platelet function: coagulation and fibrinolysis were studied before and during the infusion.

Platelet adhesiveness, as measured by a cellophane membrane test-cell system, was significantly diminished by PGE2, a change which was not noted by the glass-bead column technique. The administration of PGE2 caused more rapid platelet disaggregation following ADP-induced aggregation but had no effect on the platelet count, collagen-induced aggregation, or platelet factor 3 activity. An increase in plasma antithrombin concentration and euglobulin lysis activity was also noted.

These results support the concept that prostaglandin E2 might have a role in the prevention of thrombosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORN G. V., CROSS M. J. THE AGGREGATION OF BLOOD PLATELETS. J Physiol. 1963 Aug;168:178–195. doi: 10.1113/jphysiol.1963.sp007185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRECKENRIDGE R. T., RATNOFF C. D. Studies on the nature of the circulating anticoagulant directed against antihemophilic factor: with notes on an assay for anthemophilic factor. Blood. 1962 Aug;20:137–149. [PubMed] [Google Scholar]
  3. Bonnar J., Davidson J. F., Pidgeon C. F., McNicol G. P., Douglas A. S. Fibrin degradation products in normal and abnormal pregnancy and parturition. Br Med J. 1969 Jul 19;3(5663):137–140. doi: 10.1136/bmj.3.5663.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlson L. A., Irion E., Orö L. Effect of infusion of prostaglandin E1 on the aggregation of blood platelets in man. Life Sci. 1968 Jan 1;7(1):85–90. doi: 10.1016/0024-3205(68)90365-2. [DOI] [PubMed] [Google Scholar]
  5. Elkeles R. S., Hampton J. R., Harrison M. J., Mitchell J. R. Prostaglandin E 1 and human platelets. Lancet. 1969 Jul 12;2(7611):111–111. doi: 10.1016/s0140-6736(69)92428-3. [DOI] [PubMed] [Google Scholar]
  6. Emmons P. R., Hampton J. R., Harrison M. J., Honour A. J., Mitchell J. R. Effect of prostaglandin E1 on platelet behaviour in vitro and in vivo. Br Med J. 1967 May 20;2(5550):468–472. doi: 10.1136/bmj.2.5550.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hassanein A. A., McNicol G. P., Douglas A. S. Relationships between platelet function tests in normal and uraemic subjects. J Clin Pathol. 1970 Jul;23(5):402–406. doi: 10.1136/jcp.23.5.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirsh J., McBride J. A., Dacie J. V. Thrombo-embolism and increased platelet adhesiveness in post-splenectomy thrombocytosis. Australas Ann Med. 1966 May;15(2):122–128. doi: 10.1111/imj.1966.15.2.122. [DOI] [PubMed] [Google Scholar]
  9. Howie P. W., Mallinson A. C., Prentice C. R., Horne C. H., McNicol G. P. Effect of combined oestrogen-progestogen oral contraceptives, oestrogen, and progestogen on antiplasmin and antithrombin activity. Lancet. 1970 Dec 26;2(7687):1329–1332. doi: 10.1016/s0140-6736(70)92358-5. [DOI] [PubMed] [Google Scholar]
  10. Howie P. W., Prentice C. R., McNicol G. P. A method of antithrombin estimation using plasma defibrinates with ancrod. Br J Haematol. 1973 Jul;25(1):101–110. doi: 10.1111/j.1365-2141.1973.tb01719.x. [DOI] [PubMed] [Google Scholar]
  11. Irion E., Blombäck M. Prostaglandins in platelet aggregation. Scand J Clin Lab Invest. 1969 Sep;24(2):141–144. doi: 10.3109/00365516909080144. [DOI] [PubMed] [Google Scholar]
  12. Karim S. M., Filshie G. M. The use of prostaglandin E 2 for therapeutic abortion. J Obstet Gynaecol Br Commonw. 1972 Jan;79(1):1–13. doi: 10.1111/j.1471-0528.1972.tb15740.x. [DOI] [PubMed] [Google Scholar]
  13. Kinlough-Rathbone R. L., Packham M. A., Mustard J. F. The effect of prostaglandin E1 on platelet function in vitro and in vivo. Br J Haematol. 1970 Nov;19(5):559–571. doi: 10.1111/j.1365-2141.1970.tb01639.x. [DOI] [PubMed] [Google Scholar]
  14. Kloeze J. Influence of prostaglandins E1 and E2 on coagulation of rat blood. Experientia. 1970 Mar 15;26(3):307–308. doi: 10.1007/BF01900113. [DOI] [PubMed] [Google Scholar]
  15. Lindsay R. M., Prentice C. R., Ferguson D., Muir W. M., McNicol G. P. A method for the measurement of platelet adhesiveness by use of dialysis membranes in a test-cell. Br J Haematol. 1973 Mar;24(3):377–389. doi: 10.1111/j.1365-2141.1973.tb01661.x. [DOI] [PubMed] [Google Scholar]
  16. MCNICOL G. P., GALE S. B., DOUGLAS A. S. In-vitro and in-vivo studies of a preparation of urokinase. Br Med J. 1963 Apr 6;1(5335):909–915. doi: 10.1136/bmj.1.5335.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marquis N. R., Vigdahl R. L., Tavormina P. A. Platelet aggregation. I. Regulation by cyclic AMP and prostaglandin E1. Biochem Biophys Res Commun. 1969 Sep 10;36(6):965–972. doi: 10.1016/0006-291x(69)90298-8. [DOI] [PubMed] [Google Scholar]
  18. Mürer E. H. Effect of prostaglandin E1 on clot retraction. Nature. 1971 Jan 8;229(5280):112–113. doi: 10.1038/229112a0. [DOI] [PubMed] [Google Scholar]
  19. PROCTOR R. R., RAPAPORT S. I. The partial thromboplastin time with kaolin. A simple screening test for first stage plasma clotting factor deficiencies. Am J Clin Pathol. 1961 Sep;36:212–219. doi: 10.1093/ajcp/36.3.212. [DOI] [PubMed] [Google Scholar]
  20. RATNOFF O. D., MENZIE C. A new method for the determination of fibrinogen in small samples of plasma. J Lab Clin Med. 1951 Feb;37(2):316–320. [PubMed] [Google Scholar]
  21. REMMERT L. F., COHEN P. P. Partial purification and properties of a proteolytic enzyme of human serum. J Biol Chem. 1949 Nov;181(1):431–448. [PubMed] [Google Scholar]
  22. Vigdahl R. L., Marquis N. R., Tavormina P. A. Platelet aggregation. II. Adenyl cyclase, prostaglandin E1, and calcium. Biochem Biophys Res Commun. 1969 Oct 22;37(3):409–415. doi: 10.1016/0006-291x(69)90930-9. [DOI] [PubMed] [Google Scholar]
  23. Weeks J. R., Sekhar N. C., Ducharme D. W. Relative activity of prostaglandins E1, A1, E2 and A2 on lipolysis, platelet aggregation, smooth muscle and the cardiovascular system. J Pharm Pharmacol. 1969 Feb;21(2):103–108. doi: 10.1111/j.2042-7158.1969.tb08205.x. [DOI] [PubMed] [Google Scholar]
  24. Wolfe S. M., Shulman N. R. Inhibition of platelet energy production and release reaction by PGE1, theophylline and cAMP. Biochem Biophys Res Commun. 1970 Oct 9;41(1):128–134. doi: 10.1016/0006-291x(70)90478-x. [DOI] [PubMed] [Google Scholar]

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