Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1973 Jul;26(7):476–479. doi: 10.1136/jcp.26.7.476

The response to phytohaemagglutinin (PHA) of lymphocytes from cancer patients

S Kumar 1, G Taylor 1
PMCID: PMC477799  PMID: 4727057

Abstract

The uptake of tritiated thymidine by lymphocytes from 100 cancer patients and 31 normal subjects was tested in the presence and absence of phytohaemagglutinin (PHA) in a medium containing autologous or homologous serum. Significant differences were found between normal subjects and cancer patients in both the time course and degree of uptake. Serum from normal individuals restored the response of lymphocytes from cancer patients towards normality whilst serum from other cancer patients had no effect. Serum from cancer patients impaired the response of normal lymphocytes.

Full text

PDF
476

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes R. D., Bishun N. P., Holliday J. Impaired lymphocyte transformation and chromosomal abnormalities in fatal granulomatosus disease of childhood. Acta Paediatr Scand. 1970 Jul;59(4):403–408. doi: 10.1111/j.1651-2227.1970.tb15535.x. [DOI] [PubMed] [Google Scholar]
  2. Bredt A. B., Mardiney M. R., Jr Reactivity of human lymphocytes to autologous and homologous serum. J Immunol. 1969 Jun;102(6):1526–1529. [PubMed] [Google Scholar]
  3. Carr M. C., Stites D. P. Reactivity of maternal lymphocytes to phytohaemagglutinin. Lancet. 1972 May 13;1(7759):1073–1074. doi: 10.1016/s0140-6736(72)91260-3. [DOI] [PubMed] [Google Scholar]
  4. Cooperband S. R., Bondevik H., Schmid K., Mannick J. A. Transformation of human lymphocytes: inhibition by homologous alpha globulin. Science. 1968 Mar 15;159(3820):1243–1244. doi: 10.1126/science.159.3820.1243. [DOI] [PubMed] [Google Scholar]
  5. Coulson A. S., Chalmers D. G. Response of human blood lymphocytes to tuberculin PPD in tissue culture. Immunology. 1967 Apr;12(4):417–429. [PMC free article] [PubMed] [Google Scholar]
  6. Ducos J., Migueres J., Colombies P., Kessous A., Poujoulet N. Lymphocyte response to P.H.A. in patients with lung cancer. Lancet. 1970 May 23;1(7656):1111–1112. doi: 10.1016/s0140-6736(70)92775-3. [DOI] [PubMed] [Google Scholar]
  7. Gatti R. A. Serum inhibitors of lymphocyte responses. Lancet. 1971 Jun 26;1(7713):1351–1352. doi: 10.1016/s0140-6736(71)91906-4. [DOI] [PubMed] [Google Scholar]
  8. Golob E. K., Israsena T., Quatrale A. C., Becker K. L. Effect of serum from cancer patients on homologous lymphocyte cultures. Cancer. 1969 Feb;23(2):306–308. doi: 10.1002/1097-0142(196902)23:2<306::aid-cncr2820230207>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  9. Hartog M., Cline M. J., Grodsky G. M. The response of human leucocyte cultures to stimulation by tuberculin and phytohaemagglutinin measured by the uptake of radioactive thymidine. Clin Exp Immunol. 1967 Mar;2(2):217–228. [PMC free article] [PubMed] [Google Scholar]
  10. Kasakura S., Lowenstein L. A plasma factor in chronic lymphocytic leukemia which stimulates DNA synthesis and blastogenesis in leukocyte cultures. Blood. 1967 Apr;29(4 Suppl):691–695. [PubMed] [Google Scholar]
  11. McIntyre O. R., Cole A. F. Variation in the response of normal lymphocytes to PHA. Int Arch Allergy Appl Immunol. 1969;35(2):105–118. doi: 10.1159/000230164. [DOI] [PubMed] [Google Scholar]
  12. Nelson H. S. Delayed hypersensitivity in cancer patients: cutaneous and in vitro lymphocyte response to specific antigens. J Natl Cancer Inst. 1969 May;42(5):765–770. [PubMed] [Google Scholar]
  13. Park S. K., Brody J. I., Wallace H. A., Blakemore W. S. Immunosuppressive effect of surgery. Lancet. 1971 Jan 9;1(7689):53–55. doi: 10.1016/s0140-6736(71)90777-x. [DOI] [PubMed] [Google Scholar]
  14. Scheurlen P. G., Schneider W., Pappas A. Inhibition of transformation of normal lymphocytes by plasma factor from patients with Hodgkin's disease and cancer. Lancet. 1971 Dec 4;2(7736):1265–1265. doi: 10.1016/s0140-6736(71)90586-1. [DOI] [PubMed] [Google Scholar]
  15. Silk M. Effect of plasma from patients with carcinoma on in vitro lymphocyte transformation. Cancer. 1967 Dec;20(12):2088–2089. doi: 10.1002/1097-0142(196712)20:12<2088::aid-cncr2820201205>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  16. Whittaker M. G., Rees K., Clark C. G. Reduced lymphocyte transformation in breast cancer. Lancet. 1971 May 1;1(7705):892–893. doi: 10.1016/s0140-6736(71)92448-2. [DOI] [PubMed] [Google Scholar]
  17. al-Sarraf M., Vaitkevicius V. K. Effect of homologous plasma on lymphocyte transformation in cancer patients and controls. Lancet. 1971 Aug 7;2(7719):314–315. doi: 10.1016/s0140-6736(71)91357-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES