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. 1973 Jul;26(7):511–516. doi: 10.1136/jcp.26.7.511

A serological method for distinguishing coagulase-negative staphylococci from micrococci

L S Nakhla 1,1
PMCID: PMC477807  PMID: 4737863

Abstract

Absorbed antisera prepared against heat-killed suspensions of a coagulase-negative staphylococcus and of a micrococcus were used in a slide-agglutination test. A collection of 176 coagulase-negative aerobic cocci, from which Staphylococcus aureus had been excluded, was classified into staphylococci and micrococci by means of the test for anaerobic utilization of glucose. Of 113 staphylococci, 99 reacted with the anti-staphylococcus serum, seven with the micrococcus serum, and seven with neither; of 63 micrococci, 55 reacted with the micrococcus serum and eight were untypable. Thus 161 of 176 strains (91%) were classifiable serologically, and in 154 of 161 instances (95%) the results of the serological and biochemical classification were in agreement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  2. Baddiley J., Brock J. H., Davison A. L., Partridge M. D. The wall composition of micrococci. J Gen Microbiol. 1968 Dec;54(3):393–396. doi: 10.1099/00221287-54-3-393. [DOI] [PubMed] [Google Scholar]
  3. Bayston R. Serological investigations in children with colonized Spitz-Holter valves. J Clin Pathol. 1972 Aug;25(8):718–720. doi: 10.1136/jcp.25.8.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chalmers A. A modification of the oxidation-fermentation test for the classification of Micrococcaceae. Med Lab Technol. 1972 Oct;29(4):379–384. [PubMed] [Google Scholar]
  5. DAVISON A. L., BADDILEY J. GLYCEROL TEICHOIC ACIDS IN WALLS OF STAPHYLOCOCCUS EPIDERMIDIS. Nature. 1964 May 30;202:874–874. doi: 10.1038/202874a0. [DOI] [PubMed] [Google Scholar]
  6. Grov A., Helgeland S. M. Immunochemical characterization of Stapylococcus epidermidis and Micrococcus cell walls. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(6):812–818. doi: 10.1111/j.1699-0463.1971.tb00116.x. [DOI] [PubMed] [Google Scholar]
  7. Hasselgren I. L., Oeding P. Antigenic studies of genus Micrococcus. 1. Agglutination. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):257–264. doi: 10.1111/j.1699-0463.1972.tb00157.x. [DOI] [PubMed] [Google Scholar]
  8. Holt R. The classification of staphylococci from colonized ventriculo-atrial shunts. J Clin Pathol. 1969 Jul;22(4):475–482. doi: 10.1136/jcp.22.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mabeck C. E. Studies in urinary tract infections. II. Urinary tract infection due to coagulase-negative staphylococci. Acta Med Scand. 1969 Jul-Aug;186(1-2):39–45. [PubMed] [Google Scholar]
  10. Mortensen N. Studies in urinary tract infections. 3. Biochemical characteristics of coagulase-negative staphylococci associated with urinary tract infections. Acta Med Scand. 1969 Jul-Aug;186(1-2):47–51. [PubMed] [Google Scholar]
  11. Nakhla L. S., al-Hussaini M. K., Shokeir A. A. Acute bacterial conjunctivitis in Assiout, Upper Egypt. A one-year study. Br J Ophthalmol. 1970 Aug;54(8):540–547. doi: 10.1136/bjo.54.8.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. OEDING P. Antigenic properties of Staphylococcus aureus. Bacteriol Rev. 1960 Dec;24(4):374–396. doi: 10.1128/br.24.4.374-396.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Quinn E. L., Cox F., Fisher M. The problem of associating coagulase-negative staphylococci with disease. Ann N Y Acad Sci. 1965 Jul 23;128(1):428–442. doi: 10.1111/j.1749-6632.1965.tb11652.x. [DOI] [PubMed] [Google Scholar]
  14. Verhoef J., Van Boven C. P., Winkler K. C. Phage-typing of coagulase-negative staphylococci. J Med Microbiol. 1972 Feb;5(1):9–19. doi: 10.1099/00222615-5-1-9. [DOI] [PubMed] [Google Scholar]
  15. Verhoef J., Winkler K. C., van Boven C. P. Characters of phages from coagulase-negative staphylococci. J Med Microbiol. 1971 Nov;4(4):413–424. doi: 10.1099/00222615-4-4-413. [DOI] [PubMed] [Google Scholar]
  16. Verhoef J., Winkler K. C., van Boven C. P. Lysogeny in coagulase-negative staphylococci. J Med Microbiol. 1971 Nov;4(4):405–412. doi: 10.1099/00222615-4-4-405. [DOI] [PubMed] [Google Scholar]
  17. Watanakunakorn C., Hamburger M. Staphylococcus epidermidis endocarditis complicating a Starr-Edwards prosthesis. A therapeutic dilemma. Arch Intern Med. 1970 Dec;126(6):1014–1018. [PubMed] [Google Scholar]

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