Abstract
An anti-aldosterone serum has been used to measure urinary aldosterone concentration. A single stage of paper chromatography provides an extract of sufficient purity. Further purification by additional chromatography in a different solvent system only improves the purity by 3% ± 8 (SD). Blanks are negligible and recovery of added aldosterone is 104% ± 10 (SD).
A simplified rapid screening assay capable of revealing gross deviations from the normal is also suggested.
The diagnostic value of the assay is indicated.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUSH I. E. Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues. Biochem J. 1952 Jan;50(3):370–378. doi: 10.1042/bj0500370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bayard F., Beitins I. Z., Kowarski A., Migeon C. J. Measurement of aldosterone secretion rate by radio-immunoassay. J Clin Endocrinol Metab. 1970 Nov;31(5):507–511. doi: 10.1210/jcem-31-5-507. [DOI] [PubMed] [Google Scholar]
- Doerr P. Thin-layer chromatography and elution of picogram amounts of estradiol. J Chromatogr. 1971 Jul 22;59(2):452–456. doi: 10.1016/s0021-9673(01)82242-1. [DOI] [PubMed] [Google Scholar]
- Farmer R. W., Roup W. G., Jr, Pellizzari E. D., Fabre L. F., Jr A rapid aldosterone radioimmunoassay. J Clin Endocrinol Metab. 1972 Jan;34(1):18–22. doi: 10.1210/jcem-34-1-18. [DOI] [PubMed] [Google Scholar]
- Haning R., McCracken J., St Cyr M., Underwood R., Williams G., Abraham G. The evolution of titer and specificity of aldosterone binding antibodies in hyperimmunized sheep. Steroids. 1972 Jul;20(1):73–88. doi: 10.1016/0039-128x(72)90119-5. [DOI] [PubMed] [Google Scholar]
- KANDEL M., GORNALL A. G. EFFECT OF GLASS SURFACES ON THE LIQUID SCINTILLATION COUNTING OF ALDOSTERONE. Can J Biochem. 1964 Dec;42:1833–1837. doi: 10.1139/o64-195. [DOI] [PubMed] [Google Scholar]
- Katz F. H., Romfh P., Smith J. A. Episodic secretion of aldosterone in supine man; relationship to cortisol. J Clin Endocrinol Metab. 1972 Jul;35(1):178–181. doi: 10.1210/jcem-35-1-178. [DOI] [PubMed] [Google Scholar]
- Mayes D., Furuyama S., Kem D. C., Nugent C. A. A radioimmunoassay for plasma aldosterone. J Clin Endocrinol Metab. 1970 May;30(5):682–685. doi: 10.1210/jcem-30-5-682. [DOI] [PubMed] [Google Scholar]
- Mikhail G., Wu C. H., Ferin M., Vande Wiele R. L. Radioimmunoassay of plasma estrone and estradiol. Steroids. 1970 Mar;15(3):333–352. doi: 10.1016/s0039-128x(70)80053-8. [DOI] [PubMed] [Google Scholar]
- Robinson R. G., Fanestil D. D. Assay of aldosterone by competitive protein binding. Acta Endocrinol Suppl (Copenh) 1970;147:275–290. doi: 10.1530/acta.0.065s275. [DOI] [PubMed] [Google Scholar]