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. 1973 Sep;26(9):660–671. doi: 10.1136/jcp.26.9.660

Mesangial cells in membranous glomerulonephritis

P A Portch 1, G Williams 1
PMCID: PMC477847  PMID: 4584728

Abstract

Renal biopsies of seven patients with membranous glomerulonephritis were examined by light, electron, and immunofluorescence microscopy. All had characteristic changes of glomerular basement membranes, typically with bright granular membranous fluorescence of IgG and variable deposits of complement. Cellularity was normal or slightly increased due largely to mesangial cell proliferation. These cells assumed a variety of appearances referred to as resting, hyperactive, and dark. Resting forms occurred in cases with normal cellularity and limited complement deposition. Hyperactive cells showed ultrastructural evidence of increased secretory and digestive activity and were associated with proliferation and more pronounced complement deposition. Dark cells, interpreted as undergoing shrinkage and degeneration, were found in small numbers in all biopsies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHURG J., GRISHMAN E., GOLSTEIN M. H., YUNIS S. L., PORUSH J. G. IDIOPATHIC NEPHROTIC SYNDROME IN ADULTS. A STUDY AND CLASSIFICATION BASED ON RENAL BIOPSIES. N Engl J Med. 1965 Jan 28;272:165–174. doi: 10.1056/NEJM196501282720401. [DOI] [PubMed] [Google Scholar]
  2. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Combes B., Shorey J., Barrera A., Stastny P., Eigenbrodt E. H., Hull A. R., Carter N. W. Glomerulonephritis with deposition of Australia antigen-antibody complexes in glomerular basement membrane. Lancet. 1971 Jul 31;2(7718):234–237. doi: 10.1016/s0140-6736(71)92572-4. [DOI] [PubMed] [Google Scholar]
  4. DIXON F. J., FELDMAN J. D., VAZQUEZ J. J. Experimental glomerulonephritis. The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis. J Exp Med. 1961 May 1;113:899–920. doi: 10.1084/jem.113.5.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOSTER C., RIAD Z. M. SOME STAINING PROPERTIES OF THE GLOMERULAR CONSTITUENTS IN NORMAL RABBITS. J Anat. 1963 Jul;97:403–407. [PMC free article] [PubMed] [Google Scholar]
  6. Fisher E. R., Sharkey D., Pardo V., Vuzevski V. Experimental renal vein constriction. Its relation to renal lesions observed in human renal vein thrombosis and the nephrotic syndrome. Lab Invest. 1968 Jun;18(6):689–699. [PubMed] [Google Scholar]
  7. GOLDBERG B., GREEN H. AN ANALYSIS OF COLLAGEN SECRETION BY ESTABLISHED MOUSE FIBROBLAST LINES. J Cell Biol. 1964 Jul;22:227–258. doi: 10.1083/jcb.22.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Giard P., Paget M., Saout J., Croccel L., Leroy J. P., Cousin P. Syndrome néphrotique par thrombose unilatérale des veines rénales d'origine traumatique. J Sci Med Lille. 1969 Feb;87(2):119–141. [PubMed] [Google Scholar]
  9. Graham R. C., Jr, Karnovsky M. J. Glomerular permeability. Ultrastructural cytochemical studies using peroxidases as protein tracers. J Exp Med. 1966 Dec 1;124(6):1123–1134. doi: 10.1084/jem.124.6.1123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harris J. D., Ehrenfeld W. K., Wylie E. J., Lee J. C. Experimental renal vein occlusion. Surg Gynecol Obstet. 1968 Mar;126(3):555–562. [PubMed] [Google Scholar]
  11. JONES D. B. The nature of scar tissue in glomerulonephritis. Am J Pathol. 1963 Feb;42:185–199. [PMC free article] [PubMed] [Google Scholar]
  12. KARK R. M., MUEHRCKE R. C., PIRANI C. L., POLLAK V. E., SHAFTER H. A. Renal vein thrombosis and the nephrotic syndrome. Am J Med. 1956 Oct;21(4):496–520. doi: 10.1016/0002-9343(56)90067-5. [DOI] [PubMed] [Google Scholar]
  13. Kawano K., Arakawa M., McCoy J., Porch J., Kimmelstiel P. Quantitative study of glomeruli. Focal glomerulonephritis and diabetic glomerulosclerosis. Lab Invest. 1969 Sep;21(3):269–275. [PubMed] [Google Scholar]
  14. LATTA H. Collagen in normal rat glomeruli. J Ultrastruct Res. 1961 Aug;5:364–373. doi: 10.1016/s0022-5320(61)80013-0. [DOI] [PubMed] [Google Scholar]
  15. LATTA H., MAUNSBACH A. B., MADDEN S. C. The centrolobular region of the renal glomerulus studied by electron microscopy. J Ultrastruct Res. 1960 Dec;4:455–472. doi: 10.1016/s0022-5320(60)80033-0. [DOI] [PubMed] [Google Scholar]
  16. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lewis M. G., Loughridge L. W., Phillips T. M. Immunological studies in nephrotic syndrome associated with extrarenal malignant disease. Lancet. 1971 Jul 17;2(7716):134–135. doi: 10.1016/s0140-6736(71)92305-1. [DOI] [PubMed] [Google Scholar]
  18. MILLER F., PALADE G. E. LYTIC ACTIVITIES IN RENAL PROTEIN ABSORPTION DROPLETS. AN ELECTRON MICROSCOPICAL CYTOCHEMICAL STUDY. J Cell Biol. 1964 Dec;23:519–552. doi: 10.1083/jcb.23.3.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mandalenakis N., Mendoza N., Pirani C. L., Pollak V. E. Lobular glomerulonephritis and membranoproliferative glomerulonephritis: a clinical and pathologic study based on renal biopsies. Medicine (Baltimore) 1971 Jul;50(4):319–355. [PubMed] [Google Scholar]
  20. McCluskey R. T. Evidence for immunologic mechanisms in several forms of human glomerular diseases. Bull N Y Acad Med. 1970 Oct;46(10):769–788. [PMC free article] [PubMed] [Google Scholar]
  21. McCluskey R. T., Vassalli P., Gallo G., Baldwin D. S. An immunofluorescent study of pathogenic mechanisms in glomerular diseases. N Engl J Med. 1966 Mar 31;274(13):695–701. doi: 10.1056/NEJM196603312741301. [DOI] [PubMed] [Google Scholar]
  22. McIntosh R. M., Tinglof B., Kaufman D., Dornfeld L., Gonick H., Smith F. G., Vernier R. L. Immunohistology in renal disease. Diagnostic, prognostic, therapeutic, and etiology value and limitations. Q J Med. 1971 Jul;40(159):385–390. [PubMed] [Google Scholar]
  23. Misra R. P., Berman L. B. Studies on glomerular basement membrane. 1. Isolation and chemical analysis of normal glomerular basement membrane. Proc Soc Exp Biol Med. 1966 Jul;122(3):705–710. doi: 10.3181/00379727-122-31232. [DOI] [PubMed] [Google Scholar]
  24. Nowoslawski A., Krawczyński K., Brzosko W. J., Madaliński K. Tissue localization of Australia antigen immune complexes in acute and chronic hepatitis and liver cirrhosis. Am J Pathol. 1972 Jul;68(1):31–56. [PMC free article] [PubMed] [Google Scholar]
  25. OMAE T., MASSON G. M., CORCORAN A. C. Experimental production of nephrotic syndrome following renal vein constriction in rats. Proc Soc Exp Biol Med. 1958 Apr;97(4):821–825. doi: 10.3181/00379727-97-23890. [DOI] [PubMed] [Google Scholar]
  26. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. RHODIN J. A. Fine structure of vascular walls in mammals with special reference to smooth muscle component. Physiol Rev Suppl. 1962 Jul;5:48–87. [PubMed] [Google Scholar]
  28. Rosen S. Membranous glomerulonephritis: current status. Hum Pathol. 1971 Jun;2(2):209–231. doi: 10.1016/s0046-8177(71)80035-7. [DOI] [PubMed] [Google Scholar]
  29. Ross R., Benditt E. P. Wound healing and collagen formation. V. Quantitative electron microscope radioautographic observations of proline-H3 utilization by fibroblasts. J Cell Biol. 1965 Oct;27(1):83–106. doi: 10.1083/jcb.27.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SHELDON H., KIMBALL F. B. Studies on cartilage. III. The occurrence of collagen within vacuoles of the golgi apparatus. J Cell Biol. 1962 Mar;12:599–613. doi: 10.1083/jcb.12.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tublin I. N. Renal vein thrombosis and the nephrotic syndrome in an adult: report of a case and review of the literature. Med Ann Dist Columbia. 1968 Oct;37(10):530–535. [PubMed] [Google Scholar]
  32. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]

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