Abstract
A new technique for detecting platelet antibodies was investigated. The serum from patients having platelet antibodies was added to normal group O compatible pooled platelet-rich plasma. Platelet aggregation induced by adrenaline and adenosine diphosphate (ADP) was significantly increased as compared to the identical platelet-rich plasma without serum. The platelet-rich plasma with serum added did not aggregate spontaneously. When an alpha-adrenergic blocking agent (tolazoline) was added, the primary wave of aggregation induced by adrenaline was equally reversed with platelet-rich plasma, with or without serum.
Although platelet antibodies by themselves did not agglutinate platelets in our test system, it is probable that platelet antibodies interact with platelets and potentiate their ability to aggregate. It is suggested that the platelet aggregometer is a useful tool for the detection of platelet antibodies in vitro.
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Selected References
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- ACKROYD J. F. The cause of thrombocytopenia in sedormid purpura. Clin Sci. 1949 Dec 19;8(4):269-89, incl pl. [PubMed] [Google Scholar]
- BORN G. V. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962 Jun 9;194:927–929. doi: 10.1038/194927b0. [DOI] [PubMed] [Google Scholar]
- Clancy R., Jenkins E., Firkin B. Qualitative platelet abnormalities in idiopathic thrombocytopenic purpura. N Engl J Med. 1972 Mar 23;286(12):622–626. doi: 10.1056/NEJM197203232861202. [DOI] [PubMed] [Google Scholar]
- Deykin D., Hellerstein L. J. The assessment of drug-dependent and isoimmune antiplatelet antibodies by the use of platelet aggregometry. J Clin Invest. 1972 Dec;51(12):3142–3153. doi: 10.1172/JCI107141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRINGTON W. J., SPRAGUE C. C., MINNICH V., MOORE C. V., AULVIN R. C., DUBACH R. Immunologic mechanisms in idiopathic and neonatal thrombocytopenic purpura. Ann Intern Med. 1953 Mar;38(3):433–469. doi: 10.7326/0003-4819-38-3-433. [DOI] [PubMed] [Google Scholar]
- HOROWITZ H. I., RAPPAPORT H. I., YOUNG R. C., FUJIMOTO M. M. CHANGE IN PLATELET FACTOR 3 AS A MEANS OF DEMONSTRATING IMMUNE REACTIONS INVOLVING PLATELETS: ITS USE AS A TEST FOR QUINIDINE-INDUCED THROMBOCYTOPENIA. Transfusion. 1965 Jul-Aug;5:336–343. doi: 10.1111/j.1537-2995.1965.tb01182.x. [DOI] [PubMed] [Google Scholar]
- Karpatkin S., Siskind G. W. In vitro detection of platelet antibody in patients with idiopathic thrombocytopenic purpura and systemic lupus erythematosus. Blood. 1969 Jun;33(6):795–812. [PubMed] [Google Scholar]
- ZUCKER M. B., LEY A. B., BORRELLI J., MAYER K., FIRMAT J. Thrombocytopenia with a circulating platelet agglutinin, platelet agglutinin, platelet lysin and clot retraction inhibitor. Blood. 1959 Feb;14(2):148–161. [PubMed] [Google Scholar]