Abstract
A method for estimating thrombin clottable fibrinogen in a large number of plasma samples is described. This method is based on measuring the optical density difference of a diluted plasma and its corresponding serum after incubation at 59°C in the presence of 10% NaCl. The method gave excellent correlation with previously described thrombin clottable estimates, but was easier to perform and more accurate when estimating friable thrombin clots.
The method is compared with measurements of heat-coagulable plasma protein described by Thorp (1967) and by Millar, Simpson, and Stalker (1971), and direct optical density measurement of plasma after thrombin treatment (Burmester, Aulton, and Horsfield, 1970).
The feasibility of automating the proposed technique together with immunoreactive fibrinogen estimates is discussed.
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Selected References
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- Burmester H. B., Aulton K., Horsfield G. I. Evaluation of a rapid method for the determination of plasma fibrinogen. J Clin Pathol. 1970 Feb;23(1):43–46. doi: 10.1136/jcp.23.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELLIS B. C., STRANSKY A. A quick and accurate method for the determination of fibronogen in plasma. J Lab Clin Med. 1961 Sep;58:477–488. [PubMed] [Google Scholar]
- Farrell G. W., Wolf P. Immunoephelometric determination of fibrinogen and its derivatives in plasma. J Immunol Methods. 1972 May;1(3):217–229. doi: 10.1016/0022-1759(72)90001-4. [DOI] [PubMed] [Google Scholar]
- Farrell G. W., Wolf P. The measurement of fibrinogen and its derivatives. 3. Thrombin clottable fibrinogen estimates in the presence of epsilon amino caproic acid, fibrinogen and fibrin degradation products. Med Lab Technol. 1971 Jul;28(3):328–336. [PubMed] [Google Scholar]
- Farrell G. W., Wolf P. The measurement of fibrinogen and its derivatives. I. Examination of methods of measurement of clottable fibrinogen. Med Lab Technol. 1971 Jul;28(3):310–318. [PubMed] [Google Scholar]
- Farrell G. W., Wolf P. The measurement of fibrinogen and its derivatives. II. Determination of total immunoreactive fibrinogen in the presence of soluble fibrin monomer complexes (cryoprofibrin complexes). Med Lab Technol. 1971 Jul;28(3):319–327. [PubMed] [Google Scholar]
- Millar H. R., Simpson J. G., Stalker A. L. An evaluation of the heat precipitation method for plasma fibrinogen estimation. J Clin Pathol. 1971 Dec;24(9):827–830. doi: 10.1136/jcp.24.9.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolf P., Farrell G. W., Walton K. W. The significance of variations in immunoreactive and clottable fibrinogen in health and following thrombosis. J Clin Pathol. 1972 Jan;25(1):36–44. doi: 10.1136/jcp.25.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]