Fig. 8.

PAX4-overexpressing islets exhibit normal calcium oscillation in response to glucose. Carbachol-stimulated (100 μmol/l) intracellular release of Ca²⁺ was measured in intact islets (a) treated with DOX or (b) not treated. As indicated by the bars, islets were exposed to Ca²⁺-deficient medium containing 2 mmol/l EGTA prior to applying 100 μmol/l carbachol. (c) Analysis of the AUC for the time of the stimulus with carbachol from experiments shown in (a) and (b). (d) Analysis of the Ca²⁺ peak amplitude in response to carbachol challenge. (n = 5–6). Representative Ca²⁺ signals in response to 11 mmol/l glucose measured in intact islets (e) treated with DOX or (f) not treated. (g) Analysis of the AUC for the last 10 min of the glucose stimulus recording shown in (e) and (f). (h) Analysis of the amplitude of the first Ca²⁺ transient in response to the glucose challenge. (i) Analysis of the basal fluorescence during the first minute of the experiments. n = 9; **p ≤ 0.01 and ***p ≤ 0.001. ΔF; fluorescence increase