Abstract
A number of mathematical models have been tested for their suitability in representing the dose response curve of a specific assay of the hormone human placental lactogen (HPL).1
A new equation Y = B[0·14 + 1/[1 + C log [1 + exp[X − D]]] where Y is the percentage activity or counts bound to the antibody and X is the HPL concentration is proposed as representing the overall shape of the curve. This model is shown to give both an accurate representation of the curve and to allow reproducible determination of an unknown over a number of occasions. A number of other models are compared. The new model allows automatic calculation of HPL concentrations from a standard curve using a computer.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burger H. G., Lee V. W., Rennie G. C. A generalized computer program for the treatment of data from competitive protein-binding assays including radioimmunoassays. J Lab Clin Med. 1972 Aug;80(2):302–312. [PubMed] [Google Scholar]
- Healy M. J. Statistical analysis of radioimmunoassay data. Biochem J. 1972 Nov;130(1):207–210. doi: 10.1042/bj1300207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Täljedal I. B., Wold S. Fit of some analytical functions to insulin radio-immunoassay standard curves. Biochem J. 1970 Sep;119(2):139–143. doi: 10.1042/bj1190139. [DOI] [PMC free article] [PubMed] [Google Scholar]