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. 1974 Jan;27(1):14–20. doi: 10.1136/jcp.27.1.14

The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody

C R Taylor 1,2, J Burns 1,2
PMCID: PMC477979  PMID: 4132252

Abstract

A method is described for the demonstration of specific immunoglobulin in plasma cells and other lymphoid cells in sections taken from routine surgical histology specimens which have been formalin fixed and paraffin embedded.

An indirect sandwich technique was employed using specific rabbit antihuman immunoglobulin antisera (anti-K, L, G, A, and M) and a swine antirabbit serum Ig G, conjugated with horseradish peroxidase. The presence of plasma cells was revealed by staining the tissue-bound peroxidase-labelled antibody, having previously stained the endogenous peroxidase a contrasting colour.

It was possible to demonstrate clearly immunoglobulin in the plasma cells of tissues processed and embedded several years previously.

Some of the potential uses of the method are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Indirect immunoenzyme techniques for the intracellular detection of antigens. Immunochemistry. 1969 Nov;6(6):825–831. doi: 10.1016/0019-2791(69)90288-2. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. Bosman C., Feldman J. D. Heterogeneity and homogeneity of immunoglobulin-forming cells. Lab Invest. 1970 Apr;22(4):309–317. [PubMed] [Google Scholar]
  4. Bosman C., Feldman J. D., Pick E. Heterogeneity of antibody-forming cells. An electron microscopic analysis. J Exp Med. 1969 May 1;129(5):1029–1044. doi: 10.1084/jem.129.5.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davey F. R., Busch G. J. Immunohistochemistry of glomerulonephritis using horseradish peroxidase and fluorescein-labeled antibody: a comparison of two technics. Am J Clin Pathol. 1970 Apr;53(4):531–536. doi: 10.1093/ajcp/53.4.531. [DOI] [PubMed] [Google Scholar]
  6. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  7. Lie J. T., Holley K. E., Kampa W. R., Titus J. L. New histochemical method for morphologic diagnosis of early stages of myocardial ischemia. Mayo Clin Proc. 1971 May;46(5):319–327. [PubMed] [Google Scholar]
  8. Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
  9. Nakane P. K. Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem. 1968 Sep;16(9):557–560. doi: 10.1177/16.9.557. [DOI] [PubMed] [Google Scholar]
  10. Petts V., Roitt I. M. Peroxidase conjugates for demonstration of tissue antibodies: evaluation of the technique. Clin Exp Immunol. 1971 Sep;9(3):407–418. [PMC free article] [PubMed] [Google Scholar]

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