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. 1974 Jan;27(1):35–39. doi: 10.1136/jcp.27.1.35

Evaluation of a solid phase radioimmunoassay (Ausria-125) for the detection of hepatitis B antigen (Australia antigen) in two clinical materials

B G Hansson 1,2, T Johnsson 1,2
PMCID: PMC477982  PMID: 4856588

Abstract

A solid-phase radioimmunoassay (Ausria-125) was compared to an immunoelectro-osmophoresis method in detecting hepatitis-B antigen (HBAg). The radioimmunoassay detected HBAg in approximately 230 times higher dilutions than the immunoelectroosmophoresis method. In cases of acute hepatitis HBAg could be detected for about 70% longer after the onset of jaundice using radioimmunoassay compared with immunoelectroosmophoresis. Of 138 patients with acute hepatitis, who were HBAg-negative by immunoelectroosmophoresis, 23 (17%) were positive by radioimmunoassay. The specificity of the positive results with this test was investigated by neutralization tests with human antiserum to HBAg.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aach R. D., Grisham J. W., Parker C. W. Detection of Australia antigen by radioimmunoassay. Proc Natl Acad Sci U S A. 1971 May;68(5):1056–1060. doi: 10.1073/pnas.68.5.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blumberg B. S., Sutnick A. I., London W. T. Hepatitis and leukemia: their relation to Australia antigen. Bull N Y Acad Med. 1968 Dec;44(12):1566–1586. [PMC free article] [PubMed] [Google Scholar]
  3. Coller J. A., Millman I., Halbherr T. C., Blumberg B. S. Radioimmunoprecipitation assay for Australia antigen, antibody, and antigen-antibody complexes. Proc Soc Exp Biol Med. 1971 Oct;138(1):249–257. doi: 10.3181/00379727-138-35872. [DOI] [PubMed] [Google Scholar]
  4. Gocke D. J., Howe C. Rapid detection of Australia antigen by counterimmunoelectrophoresis. J Immunol. 1970 Apr;104(4):1031–1034. [PubMed] [Google Scholar]
  5. Hacker E. J., Jr, Aach R. D. Detection of heaptitis-associated antigen and anti-HAA. Comparison of radioimmunoassay and counterimmunoelectrophoresis. JAMA. 1973 Jan 22;223(4):414–417. [PubMed] [Google Scholar]
  6. Hansson B. G., Johnsson T. Comparative sensitivity o f immunoelectroosmophoresis and immune adherence hemagglutination tests for the detection of Australia antigen. Vox Sang. 1973;24(Suppl):95–101. doi: 10.1111/j.1423-0410.1973.tb03522.x. [DOI] [PubMed] [Google Scholar]
  7. Hansson B. G., Johnsson T. Improved technique for detecting Australia antigen by immunoelectroosmophoresis. Vox Sang. 1971 Dec;21(6):531–539. doi: 10.1111/j.1423-0410.1971.tb04812.x. [DOI] [PubMed] [Google Scholar]
  8. Hollinger F. B., Vorndam V., Dreesman G. R. Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with the passive hemagglutination methods. J Immunol. 1971 Oct;107(4):1099–1111. [PubMed] [Google Scholar]
  9. Lewis J. H., Coram J. E. Australin antigen detection. Comparison of results obtained with five CEP and one RIA test systems. Transfusion. 1972 Sep-Oct;12(5):301–305. doi: 10.1111/j.1537-2995.1972.tb04448.x. [DOI] [PubMed] [Google Scholar]
  10. Ling C. M., Overby L. R. Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody. J Immunol. 1972 Oct;109(4):834–841. [PubMed] [Google Scholar]
  11. Mayumi M., Okochi K., Nishioka K. Detection of Australia antigen by means of immune adherence haemagglutination test. Vox Sang. 1971 Feb;20(2):178–181. doi: 10.1111/j.1423-0410.1971.tb00549.x. [DOI] [PubMed] [Google Scholar]
  12. Okochi K., Mayumi M., Haguino Y., Saito N. Evaluation of frequency of Australia antigen in blood donors of Tokyo by means of immune adherence hemagglutination technique. Vox Sang. 1970 Sep-Oct;19(3):332–337. [PubMed] [Google Scholar]
  13. Pesendorfer F., Krassnitzky O., Wewalka F. Immunelektrophoretischer Nachweis von "Hepatitis-Associated-Antigen" (Au-SH-Antigen. Klin Wochenschr. 1970 Jan 1;48(1):58–59. doi: 10.1007/BF01486135. [DOI] [PubMed] [Google Scholar]
  14. Prince A. M. An antigen detected in the blood during the incubation period of serum hepatitis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):814–821. doi: 10.1073/pnas.60.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Prince A. M., Brotman B., Jass D., Ikram H. Specificity of the direct solid-phase radioimmunoassay for detection of hepatitis-B antigen. Lancet. 1973 Jun 16;1(7816):1346–1350. doi: 10.1016/s0140-6736(73)91674-7. [DOI] [PubMed] [Google Scholar]
  16. Prince A. M., Burke K. Serum hepatitis antigen (SH): rapid detection by high voltage immunoelectroosmophoresis. Science. 1970 Aug 7;169(3945):593–595. doi: 10.1126/science.169.3945.593. [DOI] [PubMed] [Google Scholar]
  17. Shulman N. R., Barker L. F. Virus-like antigen, antibody, and antigen-antibody complexes in hepatitis measured by complement fixation. Science. 1969 Jul 18;165(3890):304–306. doi: 10.1126/science.165.3890.304. [DOI] [PubMed] [Google Scholar]
  18. Vyas G. N., Shulman N. R. Hemagglutination assay for antigen and antibody associated with viral hepatitis. Science. 1970 Oct 16;170(3955):332–333. doi: 10.1126/science.170.3955.332. [DOI] [PubMed] [Google Scholar]
  19. Walsh J. H., Yalow R., Berson S. A. Detection of Australia antigen and antibody by means of radioimmunoassay techniques. J Infect Dis. 1970 May;121(5):550–554. doi: 10.1093/infdis/121.5.550. [DOI] [PubMed] [Google Scholar]

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