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. 1974 Jan;27(1):70–73. doi: 10.1136/jcp.27.1.70

A simple method for detecting mycoplasma infection of cell cultures

G B Harnett 1,2, P A Phillips 1,2, E M Mackay-Scollay 1,2
PMCID: PMC477990  PMID: 4132254

Abstract

This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

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Selected References

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  1. BARILE M. F., MALIZIA W. F., RIGGS D. B. Incidence and detection of pleuropneumonia-like organisms in cell cultures by fluorescent antibody and cultural procedures. J Bacteriol. 1962 Jul;84:130–136. doi: 10.1128/jb.84.1.130-136.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BUTLER M., LEACH R. H. A MYCOPLASMA WHICH INDUCES ACIDITY AND CYTOPATHIC EFFECT IN TISSUE CULTURE. J Gen Microbiol. 1964 Feb;34:285–294. doi: 10.1099/00221287-34-2-285. [DOI] [PubMed] [Google Scholar]
  3. Barile M. F., Kern J. Isolation of Mycoplasma arginini from commercial bovine sera and its implication in contaminated cell cultures. Proc Soc Exp Biol Med. 1971 Nov;138(2):432–437. doi: 10.3181/00379727-138-35913. [DOI] [PubMed] [Google Scholar]
  4. Cross G. F., Goodman M. R., Chatterji J., Beswick T. S., Chapman J. A. Another case of mistaken identity: rubella and mycoplasma. J Gen Virol. 1970 Jul;8(1):77–81. doi: 10.1099/0022-1317-8-1-77. [DOI] [PubMed] [Google Scholar]
  5. FOGH J., FOGH H. A METHOD FOR DIRECT DEMONSTRATION OF PLEUROPNEUMONIA-LIKE ORGANISMS IN CULTURED CELLS. Proc Soc Exp Biol Med. 1964 Dec;117:899–901. doi: 10.3181/00379727-117-29731. [DOI] [PubMed] [Google Scholar]
  6. FOGH J., FOGH H. CHROMOSOME CHANGES IN PPLO-INFECTED FL HUMAN AMNION CELLS. Proc Soc Exp Biol Med. 1965 May;119:233–238. doi: 10.3181/00379727-119-30145. [DOI] [PubMed] [Google Scholar]
  7. FOGH J., LUND R. O. Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane. Proc Soc Exp Biol Med. 1957 Mar;94(3):532–537. doi: 10.3181/00379727-94-23003. [DOI] [PubMed] [Google Scholar]
  8. Girardi A. J., Hamparian V. V., Somerson N. L., Hayflick L. Mycoplasma isolates from primary cell cultures and human diploid cell strains. Proc Soc Exp Biol Med. 1965 Dec;120(3):760–770. doi: 10.3181/00379727-120-30649. [DOI] [PubMed] [Google Scholar]
  9. Good N. E., Winget G. D., Winter W., Connolly T. N., Izawa S., Singh R. M. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467–477. doi: 10.1021/bi00866a011. [DOI] [PubMed] [Google Scholar]
  10. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  11. Levine E. M. Mycoplasma contamination of animal cell cultures: a simple, rapid detection method. Exp Cell Res. 1972 Sep;74(1):99–109. doi: 10.1016/0014-4827(72)90484-3. [DOI] [PubMed] [Google Scholar]
  12. Manchee R. J., Taylor-Robinson D. Enhanced growth of T-strain mycoplasmas with N-2-hydroxyethylpiperazone-N'-2-ethanesulfonic acid buffer. J Bacteriol. 1969 Oct;100(1):78–85. doi: 10.1128/jb.100.1.78-85.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. O'CONNELL R. C., WITTLER R. G., FABER J. E. AEROSOLS AS A SOURCE OF WIDESPREAD MYCOPLASMA CONTAMINATION OF TISSUE CULTURES. Appl Microbiol. 1964 Jul;12:337–342. doi: 10.1128/am.12.4.337-342.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Paton G. R., Jacobs J. P., Perkins F. T. Chromosome changes in human diploid-cell cultures infected with Mycoplasma. Nature. 1965 Jul 3;207(992):43–45. doi: 10.1038/207043a0. [DOI] [PubMed] [Google Scholar]
  15. Singer S. H., Ford M., Barile M., Kirschstein R. L. Effect of mycoplasmas on virus replication and plaque formation in mouse cells. Proc Soc Exp Biol Med. 1972 Jan;139(1):56–58. doi: 10.3181/00379727-139-36075. [DOI] [PubMed] [Google Scholar]
  16. Spendlove R. S., Crosbie R. B., Hayes S. F., Keeler R. F. TRICINE-buffered tissue culture media for control of mycoplasma contaminants. Proc Soc Exp Biol Med. 1971 May;137(1):258–263. doi: 10.3181/00379727-137-35556. [DOI] [PubMed] [Google Scholar]
  17. Stewart S. M., Young D. Isolation of Mycoplasma arginini from tissue-cultures. Lancet. 1971 Feb 13;1(7694):347–347. doi: 10.1016/s0140-6736(71)91072-5. [DOI] [PubMed] [Google Scholar]
  18. Surman P. G. Cytology of "pink-eye" of sheep, including a reference to trachoma of man, by employing acridine orange and iodine stains, and isolation of Mycoplasma agents from infected sheep eyes. Aust J Biol Sci. 1968 Jun;21(3):447–467. doi: 10.1071/bi9680447. [DOI] [PubMed] [Google Scholar]

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