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. 1993 Nov 15;90(22):10484–10488. doi: 10.1073/pnas.90.22.10484

Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast.

S E Plon 1, K A Leppig 1, H N Do 1, M Groudine 1
PMCID: PMC47801  PMID: 8248134

Abstract

In a screen designed to isolate human cDNAs that complement a yeast G2 phase checkpoint mutation (mec1), we isolated a cDNA homologous to the Saccharomyces cerevisiae CDC34 gene. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and represents a mammalian homolog of the group of yeast genes required for the late G1-->S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot analysis reveals evidence for a single gene that is highly conserved in higher eukaryotes. The human gene is located on the far telomeric region of 19p13.3 in a location that defines a region of homology between human chromosome 19p and mouse chromosome 11.

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