Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1974 Feb;27(2):118–121. doi: 10.1136/jcp.27.2.118

The detection of autoantibodies with an enzyme bridge method

P M Ford 1,2, P J Stoward 1,2
PMCID: PMC478018  PMID: 4596415

Abstract

The horseradish peroxidase bridge method described by Mason, Phifer, Spicer, Swallow, and Dreskin (1969) has been found to be as sensitive and specific as fluorescence methods for detecting antinuclear factor and gastric parietal cell antibody, and to a lesser extent, antibodies to smooth muscle and mitochondria. However, the method has at least four practical advantages over fluorescence techniques.

Full text

PDF
118

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Choi Y. S., Good R. A. Development of chicken lymphoid system. II. Synthesis of primordial immunoglobulin M by the bursa cells of chick embryo. J Exp Med. 1972 Jul 1;136(1):8–20. doi: 10.1084/jem.136.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  3. Hoedemaeker P. J., Feenstra K., Nijkeuter A., Arends A. Ultrastructural localization of heterologous nephrotoxic antibody in the glomerular basement membrane of the rat. Lab Invest. 1972 May;26(5):610–613. [PubMed] [Google Scholar]
  4. Mason T. E., Phifer R. F., Spicer S. S., Swallow R. A., Dreskin R. B. An immunoglobulin-enzyme bridge method for localizing tissue antigens. J Histochem Cytochem. 1969 Sep;17(9):563–569. doi: 10.1177/17.9.563. [DOI] [PubMed] [Google Scholar]
  5. Willingham M. C., Spicer S. S., Graber C. D. Immunocytologic labeling of calf and human lymphocyte surface antigens. Lab Invest. 1971 Sep;25(3):211–219. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES