Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1974 Feb;27(2):153–161. doi: 10.1136/jcp.27.2.153

Evaluation of new kits for the assessment in vitro of thyroid function by determination of serum total thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and 125I-labelled triiodothyronine and thyroxine

P J N Howorth 1,2, C G McKerron 1,2
PMCID: PMC478032  PMID: 4132967

Abstract

A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combined to give a free thyroxine index (Trilute-Tetralute-FTI) increased further the diagnostic discrimination and usefulness of the tests.

The method for the determination of serum thyroxine can be modified to give a direct estimate of serum free thyroxine, expressed as a free thyroxine index. This new single-column technique, called the `single-column free thyroxine index', gave a good correlation with clinical thyroid status in a preliminary trial of 45 patients.

Full text

PDF
153

Images in this article

157Image
on p.157
158Image
on p.158
158Image
on p.158
159Image
on p.159
160Image
on p.160

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braverman L. E., Vagenakis A. G., Foster A. E., Ingbar S. H. Evaluation of a simplified technique for the specific measurement of serum thyroxine concentration. J Clin Endocrinol Metab. 1971 Apr;32(4):497–502. doi: 10.1210/jcem-32-4-497. [DOI] [PubMed] [Google Scholar]
  2. CLARK F., HORN D. B. ASSESSMENT OF THYROID FUNCTION BY THE COMBINED USE OF THE SERUM PROTEIN-BOUND IODINE AND RESIN UPTAKE OF 131-I-TRIIODOTHYRONINE. J Clin Endocrinol Metab. 1965 Jan;25:39–45. doi: 10.1210/jcem-25-1-39. [DOI] [PubMed] [Google Scholar]
  3. CUARON A., FUCUGAUCHI M. E. THE BINDING OF 131-I-TRIIODOTHYRONINE BY SERUM PROTEINS AS AN IN VITRO TEST OF THYROID FUNCTION. Acta Endocrinol (Copenh) 1964 Jun;46:161–169. doi: 10.1530/acta.0.0460161. [DOI] [PubMed] [Google Scholar]
  4. Gimlette T. M. Use of Sephadex column chromatography in the assessment of thyroid status. J Clin Pathol. 1967 Mar;20(2):170–174. doi: 10.1136/jcp.20.2.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howorth P. J., Maclagan N. F. Clinical application of serum-total-thyroxine estimation, resin uptake, and free-thyroxine index. Lancet. 1969 Feb 1;1(7588):224–228. doi: 10.1016/s0140-6736(69)91240-9. [DOI] [PubMed] [Google Scholar]
  6. Hvid Hansen H. Sephadex binding of 131-I-labelled L-triiodo-thyronine as a test of thyroid function. Scand J Clin Lab Invest. 1966;18(2):240–244. doi: 10.3109/00365516609051820. [DOI] [PubMed] [Google Scholar]
  7. LEE N. D., HENRY R. J., GOLUB O. J. DETERMINATION OF THE FREE THYROXINE CONTENT OF SERUM. J Clin Endocrinol Metab. 1964 Jun;24:486–495. doi: 10.1210/jcem-24-6-486. [DOI] [PubMed] [Google Scholar]
  8. LIEWENDAHL K., LAMBERG B. A. FREE THYROXINE IN SERUM DETERMINED BY DIALYSIS AND SEPHADEX G-25 FILTRATION. J Clin Endocrinol Metab. 1965 Jul;25:991–993. doi: 10.1210/jcem-25-7-991. [DOI] [PubMed] [Google Scholar]
  9. LISSITZKY S., BISMUTH J. [Quantitative determination of the concentration of I-131-thyroxin and I-131-triodothyronine in the serum by filtration on dextran gel (Sephadex)]. Clin Chim Acta. 1963 Mar;8:269–276. doi: 10.1016/0009-8981(63)90167-0. [DOI] [PubMed] [Google Scholar]
  10. MOUGEY E. H., MASON J. W. SEPARATION OF SOME IODOAMINO ACIDS AND IODIDE BY GEL FILTRATION. Anal Biochem. 1963 Sep;6:223–233. doi: 10.1016/0003-2697(63)90129-5. [DOI] [PubMed] [Google Scholar]
  11. MURPHY B. E., PATTEE C. J. DETERMINATION OF THYROXINE UTILIZING THE PROPERTY OF PROTEIN-BINDING. J Clin Endocrinol Metab. 1964 Feb;24:187–196. doi: 10.1210/jcem-24-2-187. [DOI] [PubMed] [Google Scholar]
  12. Maclagan N. F., Howorth P. J. Thyroid function studies using resin uptake of radioactive thyronines from serum and total thyroxine assay; the free thyroxine index. Clin Sci. 1969 Aug;37(1):45–60. [PubMed] [Google Scholar]
  13. Mincey E. K., Thorson S. C., Brown J. L. A new in-vitro blood test for determining thyroid status: the effective thyroxine ratio. Clin Biochem. 1971 Dec;4(4):216–221. doi: 10.1016/s0009-9120(71)91645-6. [DOI] [PubMed] [Google Scholar]
  14. Murphy B. E., Pattee C. J., Gold A. Clinical evaluation of a new method for the determination of serum thyroxine. J Clin Endocrinol Metab. 1966 Mar;26(3):247–256. doi: 10.1210/jcem-26-3-247. [DOI] [PubMed] [Google Scholar]
  15. OSORIO C., JACKSON D. J., GARTSIDE J. M., GOOLDEN A. W. The assessment of free thyroxine in plasma. Clin Sci. 1962 Dec;23:525–530. [PubMed] [Google Scholar]
  16. PORATH J., FLODIN P. Gel filtration: a method for desalting and group separation. Nature. 1959 Jun 13;183(4676):1657–1659. doi: 10.1038/1831657a0. [DOI] [PubMed] [Google Scholar]
  17. Seligson H., Seligson D. Measurement of thyroxine by competitive protein binding. Clin Chim Acta. 1972 Apr;38(1):199–205. doi: 10.1016/0009-8981(72)90226-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES