Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1974 Apr;27(4):315–318. doi: 10.1136/jcp.27.4.315

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: A biochemical investigation

M I Filipe 1,2, K B Cooke 1,2
PMCID: PMC478111  PMID: 4850957

Abstract

Fifteen surgical specimens from patients with carcinoma of the colon and rectum were studied. Scrapings from normal mucosa distant from the tumour and from macroscopically normal mucosa adjacent to the tumour (`transitional') were used for chemical estimation of hexosamines, sialic acid, and proteins. The presence of hexosamines and sialic acid was confirmed in both normal and transitional mucosa. Transitional mucosa showed increased levels of total hexosamines and sialic acid as compared with the normal and this was accompanied by an increase in neuraminidase-sensitive sialic acids. The present data have been compared with previous histochemical and autoradiographic studies and it is suggested that the changes described in the transitional mucosa are transformations representing an early stage of carcinogenesis.

Full text

PDF
315

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANTONOPOULOS C. A., GARDELL S., SZIRMAI J. A., DETYSSONSK E. R. DETERMINATION OF GLYCOSAMINOGLYCANS (MUCOPOLYSACCHARIDES) FROM TISSUE ON THE MICROGRAM SCALE. Biochim Biophys Acta. 1964 Mar 2;83:1–19. doi: 10.1016/0926-6526(64)90045-x. [DOI] [PubMed] [Google Scholar]
  3. Allen A., Kent P. W. Biosynthesis of intestinal mucins. Effect of puromycin on mucoprotein biosynthesis by sheep colonic mucosal tissue. Biochem J. 1968 Jan;106(1):301–309. doi: 10.1042/bj1060301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Andrews A. T., Herring G. M., Kent P. W. Some studies on the composition of bovine cortical-bone sialoprotein. Biochem J. 1967 Sep;104(3):705–715. doi: 10.1042/bj1040705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BARKER S. A., STACEY M., TIPPER D. J. Some observations on certain mucorproteins containing neuraminic acid. Nature. 1959 Sep 5;184:68–69. doi: 10.1038/184068a0b. [DOI] [PubMed] [Google Scholar]
  6. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  7. Burdette W. J. Carcinoma of the colon and antecedent epithelium. Cancer Res. 1970 Jan;30(1):253–256. [PubMed] [Google Scholar]
  8. DESCHNER E., LEWIS C. M., LIPKIN M. IN VITRO STUDY OF HUMAN RECTAL EPITHELIAL CELLS. I. ATYPICAL ZONE OF H3 THYMIDINE INCORPORATION IN MUCOSA OF MULTIPLE POLYPOSIS. J Clin Invest. 1963 Dec;42:1922–1928. doi: 10.1172/JCI104878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Draper P., Kent P. W. Biosynthesis of intestinal mucins. 4. Utilization of [1-C]glucose by sheep colonic mucosa in vitro. Biochem J. 1963 Feb;86(2):248–254. doi: 10.1042/bj0860248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Esterly J. R., Spicer S. S. Mucin histochemistry of human gallbladder: changes in adenocarcinoma, cystic fibrosis, and cholecystitis. J Natl Cancer Inst. 1968 Jan;40(1):1–12. [PubMed] [Google Scholar]
  11. Filipe M. I. 35 sulphur uptake in the mucosa adjacent to carcinoma of the large intestine. Histochem J. 1971 Jan;3(1):27–35. doi: 10.1007/BF01686504. [DOI] [PubMed] [Google Scholar]
  12. Filipe M. I. The value of a study of the mucosubstances in rectal biopsies from patients with carcinoma of the rectum and lower sigmoid in the diagnosis of premalignant mucosa. J Clin Pathol. 1972 Feb;25(2):123–128. doi: 10.1136/jcp.25.2.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Filipe M. I. Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut. 1969 Jul;10(7):577–586. doi: 10.1136/gut.10.7.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. GOLD P., FREEDMAN S. O. DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES. J Exp Med. 1965 Mar 1;121:439–462. doi: 10.1084/jem.121.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GURDON J. B. Adult frogs derived from the nuclei of single somatic cells. Dev Biol. 1962 Apr;4:256–273. doi: 10.1016/0012-1606(62)90043-x. [DOI] [PubMed] [Google Scholar]
  16. Griffen W. O., Jr, Meeker W. R. Colon carcinoma and immunologic phenomena. Surg Clin North Am. 1972 Aug;52(4):839–846. doi: 10.1016/s0039-6109(16)39783-3. [DOI] [PubMed] [Google Scholar]
  17. Imondi A. R., Balis M. E., Lipkin M. Changes in enzyme levels accompanying differentiation of intestinal epithelial cells. Exp Cell Res. 1969 Dec;58(2):323–330. doi: 10.1016/0014-4827(69)90512-6. [DOI] [PubMed] [Google Scholar]
  18. Imondi A. R., Lipkin M., Balis M. E. Enzyme and template stability as regulatory mechanisms in differentiating intestinal epithelial cells. J Biol Chem. 1970 May 10;245(9):2194–2198. [PubMed] [Google Scholar]
  19. Korhonen L. K., Mäkelä V. Carbohydrate-rich compounds in the colonic mucosa of man. 3. A preliminary report of some biochemical characteristics of the carbohydrate-rich components in normal colonic mucosa. Histochem J. 1971 Mar;3(2):101–106. doi: 10.1007/BF01003055. [DOI] [PubMed] [Google Scholar]
  20. Krupey J., Gold P., Freedman S. O. Physicochemical studies of the carcinoembryonic antigens of the human digestive system. J Exp Med. 1968 Sep 1;128(3):387–398. doi: 10.1084/jem.128.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LIPKIN M. CELL REPLICATION IN THE GASTROINTESTINAL TRACT OF MAN. Gastroenterology. 1965 May;48:616–624. [PubMed] [Google Scholar]
  22. LIPKIN M., OUASTLER H. Cell retention and incidence of carcinoma in several portions of the gastrointestinal tract. Nature. 1962 Jun 23;194:1198–1199. doi: 10.1038/1941198a0. [DOI] [PubMed] [Google Scholar]
  23. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  24. Langvad E. Lactate dehydrogenase isoenzyme patterns in the tumour-bearing colon. Int J Cancer. 1968 Jan 15;3(1):17–29. doi: 10.1002/ijc.2910030104. [DOI] [PubMed] [Google Scholar]
  25. Lee A. K., Rowley M., Mackay I. R. Antibody-producing capacity in human cancer. Br J Cancer. 1970 Sep;24(3):454–463. doi: 10.1038/bjc.1970.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Lev R. The mucin histochemistry of normal and neoplastic gastric mucosa. Lab Invest. 1965 Dec;14(12):2080–2100. [PubMed] [Google Scholar]
  27. Lewis B., Morson B. C., February A. W., Jones J. H., Misiewicz J. J. Abnormal lactic dehydrogenase isoenzyme patterns in ulcerative colitis with precancerous change. Gut. 1971 Jan;12(1):16–19. doi: 10.1136/gut.12.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Lipkin M., Deschner E., Troncale F. Cell differentiation and the development of colonic neoplasms. Gastroenterology. 1970 Aug;59(2):303–307. [PubMed] [Google Scholar]
  29. Lipkin M. Newer measurements of cell proliferation in the colon. Gastroenterology. 1966 Nov;51(5):851–854. [PubMed] [Google Scholar]
  30. Lo Gerfo P., Herter F. P., Barker H. G., Bennett S. Immunologic tests for the detection of gastrointestinal cancers. Surg Clin North Am. 1972 Aug;52(4):829–837. doi: 10.1016/s0039-6109(16)39782-1. [DOI] [PubMed] [Google Scholar]
  31. Nairn R. C., Fothergill J. E., McEntegart M. G., Richmond H. G. Loss of Gastro-intestinal-specific Antigen in Neoplasia. Br Med J. 1962 Jun 30;1(5295):1791–1793. doi: 10.1136/bmj.1.5295.1791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. PASTERNAK C. A., KENT P. W. Biosynthesis of intestinal mucins. 2. Incorporation of [35S] sulphate by guinea-pig colon in vitro. Biochem J. 1958 Mar;68(3):452–457. doi: 10.1042/bj0680452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sky-Peck H. H., Lundgren R., Bornstein I. Acid mucopolysaccharide composition in human colon. Ann N Y Acad Sci. 1966 Sep 30;130(3):951–962. doi: 10.1111/j.1749-6632.1966.tb12639.x. [DOI] [PubMed] [Google Scholar]
  34. Stonehill E. H., Bendich A. Retrogenetic expression: the reappearance of embryonal antigens in cancer cells. Nature. 1970 Oct 24;228(5269):370–372. doi: 10.1038/228370a0. [DOI] [PubMed] [Google Scholar]
  35. Troncale F., Hertz R., Lipkin M. Nucleic acid metabolism in proliferating and differentiating colonic cells of man and in neoplastic lesions of the colon. Cancer Res. 1971 Apr;31(4):463–467. [PubMed] [Google Scholar]
  36. WERNER I. Studies on glycoproteins from mucous epithelium and epithelial secretions. Acta Soc Med Ups. 1953;58(1-2):1–55. [PubMed] [Google Scholar]
  37. von Kleist S., Burtin P. Isolation of a fetal antigen from human colonic tumors. Cancer Res. 1969 Nov;29(11):1961–1964. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES