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. 1974 May;27(5):377–381. doi: 10.1136/jcp.27.5.377

Conventional and dual competitive binding analyses of thyroxine using the Thyopac-4 kit

Satoshi Hamada 1,2,3, Kanji Torizuka 1,2,3, Tetsuo Uete 1,2,3
PMCID: PMC478130  PMID: 4211892

Abstract

A simple method is described for the simultaneous determinations of thyroxine and the effective thyroxine ratio (ETR) using a single kit of Thyopac-4. Thyopac ETR values obtained were 1·00 ± 0·03 for normal subjects; 1·22 ± 0·11 for hyperthyroid; 0·87 ± 0·04 for hypothyroid; and 1·02 ± 0·05 for pregnant women. Cases with TBG deficiency and the nephrotic syndrome indicated normal values. The value (x) was correlated significantly (γ = + 0·961) with the free Thyopac index (y), representing a regression equation of y = 71·7x2 − 88·4x + 24·2. Thyopac ETR was shown to be useful in differentiating normal thyroid states from abnormal, irrespective of changes in T4-binding globulin (TBG). Further, the T4 value obtained simultaneously made it possible to detect changes in the TBG level by noting an inconsistent value for the effective thyroxine ratio.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashkar F. S., Bezjian A. A. Use of normalized serum thyroxine (T 4 N). A new approach to thyroid hormone measurement. JAMA. 1972 Sep 25;221(13):1483–1485. [PubMed] [Google Scholar]
  2. CLARK F., HORN D. B. ASSESSMENT OF THYROID FUNCTION BY THE COMBINED USE OF THE SERUM PROTEIN-BOUND IODINE AND RESIN UPTAKE OF 131-I-TRIIODOTHYRONINE. J Clin Endocrinol Metab. 1965 Jan;25:39–45. doi: 10.1210/jcem-25-1-39. [DOI] [PubMed] [Google Scholar]
  3. Clark F., Brown H. J. Evaluation of Thyopac-3 test in the in-vitro assessment of thyroid function. Br Med J. 1970 Mar 21;1(5698):713–715. doi: 10.1136/bmj.1.5698.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark F., Brown H. J. Free thyroxine index. Br Med J. 1970 Jun 13;2(5710):672–672. doi: 10.1136/bmj.2.5710.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GOOLDEN A. W., GARTSIDE J. M., OSORIO C. AN EVALUATION OF THE 131-I-TRIIODOTHYRONINE RESIN SPONGE TEST. J Clin Endocrinol Metab. 1965 Jan;25:127–133. doi: 10.1210/jcem-25-1-127. [DOI] [PubMed] [Google Scholar]
  6. Goolden A. W., Gartside J. M., Sanderson C. Thyroid status in pregnancy and in women taking oral contraceptives. Lancet. 1967 Jan 7;1(7480):12–15. doi: 10.1016/s0140-6736(67)92420-8. [DOI] [PubMed] [Google Scholar]
  7. Hamada S., Nakagawa T., Mori T., Torizuka K. Re-evaluation of thyroxine binding and free thyroxine in human serum by paper electrophoresis and equilibrium dialysis, and a new free thyroxine index. J Clin Endocrinol Metab. 1970 Aug;31(2):166–179. doi: 10.1210/jcem-31-2-166. [DOI] [PubMed] [Google Scholar]
  8. INGBAR S. H., FREINKEL N. Regulation of the peripheral metabolism of the thyroid hormones. Recent Prog Horm Res. 1960;16:353–403. [PubMed] [Google Scholar]
  9. Inada M., Okabe J., Kazawa Y., Takayama H., Haruna M. [Thyroid function test by Res-O-Mat ETR test. II. Simultaneous determination of the effective thyroxine ratio and thyroxine concentration in the blood Res-O-Mat ETR test]. Kaku Igaku. 1973 Feb;10(1):37–43. [PubMed] [Google Scholar]
  10. Mincey E. K., Thorson S. C., Brown J. L., Morrison R. T., McIntosh H. W. A new parameter of thyroid function--the effective thyroxine ratio. J Nucl Med. 1972 Feb;13(2):165–168. [PubMed] [Google Scholar]
  11. ROBBINS J., RALL J. E. Proteins associated with the thyroid hormones. Physiol Rev. 1960 Jul;40:415–489. doi: 10.1152/physrev.1960.40.3.415. [DOI] [PubMed] [Google Scholar]

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