Abstract
A one-day procedure for detecting salmonellae was developed and evaluated with clinical faecal specimens. The procedure was based on the selective retardation of enterobacteria in their migration through a semi-solid enrichment medium. It was shown that a pure or almost pure growth of salmonella could often be obtained within 16 to 24 hours by this method. The culture thus obtained could then be used directly for serological confirmation. The method was rapid, economical, and efficient. Clinical trials indicated an increase of 28% in the frequency of positive isolations of salmonella with this procedure as compared with the conventional multistep cultural procedures with no false-positive results. However, the method was unsatisfactory for detecting Salmonella typhi due to its slow migration through the semi-solid medium.
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Selected References
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- Banwart G. J. Glassware apparatus for determining motile bacteria. 1. Salmonella. Poult Sci. 1968 Jul;47(4):1209–1212. doi: 10.3382/ps.0471209. [DOI] [PubMed] [Google Scholar]
- Fung D. Y., Kraft A. A. A rapid and simple method for the detection and isolation of Salmonella from mixed cultures and poultry products. Poult Sci. 1970 Jan;49(1):46–54. doi: 10.3382/ps.0490046. [DOI] [PubMed] [Google Scholar]
- Gibbs P. A., Patterson J. T., Murray J. G. The fluorescent antibody technique for the detection of Salmonella in routine use. J Appl Bacteriol. 1972 Sep;35(3):405–413. doi: 10.1111/j.1365-2672.1972.tb03716.x. [DOI] [PubMed] [Google Scholar]
- Goepfert J. M., Mann M. E., Hicks R. One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods. Appl Microbiol. 1970 Dec;20(6):977–983. doi: 10.1128/am.20.6.977-983.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harper J., Shortridge K. F. A selective motility medium for routine isolation of Salmonella. J Hyg (Lond) 1969 Jun;67(2):181–186. doi: 10.1017/s0022172400041589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iveson J. B., Mackay-Scollay E. M. An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents. J Hyg (Lond) 1972 Jun;70(2):367–384. doi: 10.1017/s0022172400022415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUART P. F., PIVNICK H. ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS. Appl Microbiol. 1965 May;13:365–372. doi: 10.1128/am.13.3.365-372.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomason B. M., McWhorter A. C. Rapid detection of typhoid carriers by means of fluorescent antibody techniques. Bull World Health Organ. 1965;33(5):681–685. [PMC free article] [PubMed] [Google Scholar]