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. 1974 May;27(5):420–426. doi: 10.1136/jcp.27.5.420

The reliability of methicillin sensitivity tests on four culture media

D F J Brown 1,2, D Kothari 1,2
PMCID: PMC478140  PMID: 4496682

Abstract

The influence of culture medium, incubation temperature, and antibiotic content on the reliability of methicillin disc-sensitivity tests on strains of Staphylococcus aureus was evaluated. Tests were performed with an inoculum giving semiconfluent growth of colonies on four media, with 5-μg and 10-μg methicillin discs at incubation temperatures of 30, 35, and 37°C. With Sensitest agar and Mueller-Hinton agar at 35°C or below, methicillin resistance was reliably detected. On DST agar and Wellcotest agar discrimination between sensitive and resistant strains was possible only at 30°C. On Wellcotest agar and DST agar it was not possible to detect methicillin resistance in one resistant strain at any temperature. For sensitive and resistant strains, the use of a 5-μg disc allowed greater differentiation than the 10-μg disc.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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