Table 2.

Top 20 metabolic pathways that have intermediate and end-product metabolites that display significant changes in intensity between planktonic cells and biofilm samples

Pathway namea	KEGG map IDa	Number of metabolitesb	Annotated (${\text{HRMS}}_{\text{PL}}^1$)c	Identified (${\text{HRMS}}_{\text{a}}^1$ Rta)d	Coveragee (%)	P vs. Bf
Arginine and proline metabolism/arginine biosynthesis	00330/00220	90	49	5	60	33
Protein digestion and absorption	04974	47	22	7	61.7	19
Tyrosine metabolism	00350	76	35	2	48.7	18
Histidine metabolism	00340	45	25	3	62.2	17
Galactose metabolism	00052	41	22	0	53.7	17
Aminoacyl-tRNA biosynthesis	00970	52	13	7	38.5	17
Cyanoamino acid metabolism	00460	46	31	1	69.6	16
Linoleic acid metabolism	00591	28	26	0	92.9	15
Limonene and pinene degradation	00903	64	53	0	82.8	15
C5-Branched dibasic acid metabolism	00660	32	20	4	75	14
Mineral absorption	04978	29	11	4	51.7	14
Phosphotransferase system (PTS)	02060	48	20	1	43.8	14
Two-component system	02020	41	10	6	39	14
Fructose and mannose metabolism	00051	51	16	0	31.4	14
Alanine, aspartate and glutamate metabolism	00250	24	15	2	70.8	13
Lysine degradation	00310	47	26	1	57.4	13
Glycine, serine and threonine metabolism	00260	51	27	2	56.9	13
Phenylalanine metabolism	00360	72	33	1	47.2	13
Pyrimidine metabolism	00240	66	28	5	50	12
Aminobenzoate degradation	00627	84	28	1	34.5	12
Purine metabolism	00230	92	16	9	27.2	12
Citrate cycle (TCA cycle)	00020	20	5	3	40	4

^aIn accordance with KEGG (Kyoto Encyclopaedia of Genes and Genomes data base) (Kanehisa and Goto 2000)

^bTotal number of metabolites in the pathway according to KEGG

^cNumber of annotated metabolites detected, metabolite code ${\text{HRMS}}_{\text{PL}}^1$ (Sumner et al. 2014)

^dNumber of identified metabolites detected, metabolite code ${\text{HRMS}}_{\text{a}}^1$ R_ta (Sumner et al. 2014)

^ePercentage of metabolites detected in the pathway (${\text{HRMS}}_{\text{PL}}^1$ and ${\text{HRMS}}_{\text{a}}^1$ R_ta)

^fNumber of metabolites detected (${\text{HRMS}}_{\text{PL}}^1$ and ${\text{HRMS}}_{\text{a}}^1$ R_ta) that show significant changes in expression profiles between Planktonic (P) and biofilm (B) samples