In the article by Yoshida et al. (Journal of Clinical Biochemistry and Nutrition 2014; 55: 90–96) “Evaluation of radical scavenging properties of shikonin”, an error appeared in Table 1. In the left ’ROS’ column, tert-butyl peroxyl radical (BuOO•) appeared in an incorrect line: singlet oxygen (1O2) should be in its place, and BuOO• should be in the last line of the column. This correction is limited to this column and does not change the conclusions of the study. The corrected Table 1 with footnote is printed below.
Table 1.
Review of the ROS-scavenging activity of shikonin
| ROS | IC50 (µM) | Assay | Reference |
|---|---|---|---|
| O2•− | 7.2 | EPR† | Sekine et al. (ref. 10) |
| 17 | EPR† | Gao et al. (ref. 12) | |
| •OH | 40§ | EPR† | Sekine et al. (ref. 11) |
| 108 | EPR† | ref. 12 | |
| 1O2 | 27 | EPR‡ | ref. 12 |
| BuOO· | 113 | EPR† | ref. 12 |
The sources of superoxide (O2•−), hydroxyl radical (•OH), singlet oxygen (1O2) and tert-butyl peroxyl radical (BuOO•) were respectively, hypoxanthine/xanthine oxidase system, Fenton reaction, hematoporphyrin irradiated with UVB, and tert-butyl hydroperoxide reacting with methemoglobin and diethylenetriamine-N,N,N',N'',N''-pentaacetic acid. †spin trap: 5,5-dimethyl-1-pyrroline-N-oxide; ‡spin trap: 2,2,6,6-tetramethyl-4-piperidone hydrochloride; §determined for shikonin and alkannin in mixture (82:18).